人p100蛋白:可增强STAT6基因转录活性的新因子

被引:5
作者
杨洁
姚智
郁春艳
Olli Silvennoinen
机构
[1] 天津医科大学免疫学教研室,天津医科大学免疫学教研室,天津医科大学免疫学教研室,芬兰坦佩雷大学医学技术研究所天津,天津,天津
关键词
STAT6; 人类p100蛋白; RNA多聚酶Ⅱ; 基因转录;
D O I
暂无
中图分类号
R392.11 [免疫分子生物学(免疫化学)];
学科分类号
100102 ;
摘要
目的 :建立可稳定表达人类p10 0蛋白的细胞株 ,研究细胞内p10 0蛋白参与STAT6介导的基因转录调控的机制。方法 :利用免疫共沉淀法检测蛋白与蛋白间结合 ,荧光素酶法测定STAT6基因转录活性。结果 :p10 0既可与STAT6结合 ,亦可与RNA多聚酶Ⅱ结合 ,并能增强STAT6介导的基因转录活性。结论 :p10 0蛋白是STAT6共激活因子 ,可桥连STAT6和基本转录调控元件 ,促进STAT6介导的基因转录活性。
引用
收藏
页码:739 / 742
页数:4
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