基于BSA-seq和RNA-seq挖掘水稻株高相关QTL

被引：3

作者：

吴元明 ^{[1
]}

林佳怡 ^{[1
]}

柳雨汐 ^{[1
]}

李丹婷 ^{[2
]}

张宗琼 ^{[2
]}

郑晓明 ^{[3
,4
,5
]}

逄洪波 ^{[1
]}

机构：

[1] 沈阳师范大学生命科学学院

[2] 广西壮族自治区农业科学院水稻研究所广西水稻遗传育种重点实验室

[3] 中国农业科学院作物科学研究所

[4] 海南三亚中国农业科学院国家南繁研究院

[5] 国际水稻研究所

来源：

生物技术通报 | 2023年 / 39卷 / 08期

基金：

海南省自然科学基金;

关键词：

水稻; 株高; 定位; BSA-seq; RNA-seq;

D O I：

10.13560/j.cnki.biotech.bull.1985.2023-0386

中图分类号：

S511 [稻];

学科分类号：

摘要：

株高是影响水稻产量稳定性的重要因素之一，水稻株高相关QTL的定位及候选基因的挖掘，有助于深入了解株高分子调控机制，进而为培育理想株型的水稻品种奠定基础。以油占8号及广西野生稻为亲本构建的285个CSSL群体为试验对象，结合NGS与BSA-seq等方法，利用SNP和InDel两种分子标记进行关联分析，对可能与株高相关的基因组区段进行定位。结果显示，Δ（SNP-index）分子标记在Chr.7和Chr.10中分别关联到3.205和1.311 Mb大小的候选基因组区域。Δ（InDel-index）标记关联到的基因组区域大小分别为2.848和1.292 Mb，且全部包含于Δ（SNP-index）关联到的区间内。结合GO、KEGG、Uniprot、eggNOG等数据库中的功能注释、高质量多态性位点筛选以及已有的株高相关转录组数据，最终关联到Chr.7中的5个候选基因，包括功能和机理已知的OsTCP21。RT-qPCR结果显示，OsTCP21在高株和矮株中的表达差异与已有研究结果相一致，LOC＿Os07g05050和LOC＿Os07g02850在高株中表达量较高，LOC＿Os07g04220和LOC＿Os07g02770在矮株中表达量较高。这5个候选基因在水稻株高性状的调控中起重要的作用，OsTCP21是一个关键调控基因。

引用

页码：173 / 184

页数：12

共 43 条

[1] The identification and characterization of a plant height and grain length related gene hfr131 in rice..[J].Lan Dengyong;Cao Liming;Liu Mingyu;Ma Fuying;Yan Peiwen;Zhang Xinwei;Hu Jian;Niu Fuan;He Shicong;Cui Jinhao;Yuan Xinyu;Yang Jinshui;Wang Ying;Luo Xiaojin.Frontiers in plant science.2023,
[2] OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine-tuning auxin transport in rice
Li, Yan
He, Yizhou
Liu, Zhixin
Qin, Tian
Wang, Lei
Chen, Zhihui
Zhang, Biaoming
Zhang, Haitao
Li, Haitao
Liu, Li
Zhang, Jian
Yuan, Wenya
[J]. PLANT JOURNAL, 2022, 111 (04): : 1167 - 1182
[3] Dwarf and High Tillering1 represses rice tillering through mediating the splicing of D14 pre-mRNA
Liu, Tianzhen
Zhang, Xin
Zhang, Huan
Cheng, Zhijun
Liu, Jun
Zhou, Chunlei
Luo, Sheng
Luo, Weifeng
Li, Shuai
Xing, Xinxin
Chang, Yanqi
Shi, Cuilan
Ren, Yulong
Zhu, Shanshan
Lei, Cailin
Guo, Xiuping
Wang, Jie
Zhao, Zhichao
Wang, Haiyang
Zhai, Huqu
Lin, Qibing
Wan, Jianmin
[J]. PLANT CELL, 2022, 34 (09): : 3301 - 3318
[4] The Kelch-F-box protein SMALL AND GLOSSY LEAVES 1 (SAGL1) negatively influences salicylic acid biosynthesis in Arabidopsis thaliana by promoting the turn-over of transcription factor SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1)
Yu, Ke
Yang, Wenqi
Zhao, Bing
Wang, Ling
Zhang, Pan
Ouyang, Yi
Chang, Yuankai
Chen, Guoqingzi
Zhang, Junli
Wang, Shujie
Wang, Xiao
Wang, Panpan
Wang, Wei
Roberts, Jeremy A.
Jiang, Kun
Mur, Luis A. J.
Zhang, Xuebin
[J]. NEW PHYTOLOGIST, 2022, 235 (03) : 885 - 897
[5] Bulk segregation analysis in NGS era: a review for its teenage years..[J].Li Zhiqiang;Xu Yuhui.The Plant journal : for cell and molecular biology.2021, 6
[6] Blocking Osa-miR1871 enhances rice resistance against Magnaporthe oryzae and yield..[J].Li Yan;Li TingTing;He XiaoRong;Zhu Yong;Feng Qin;Yang XueMei;Zhou XinHui;Li GuoBang;Ji YunPeng;Zhao JingHao;Zhao ZhiXue;Pu Mei;Zhou ShiXin;Zhang JiWei;Huang YanYan;Fan Jing;Wang WenMing.Plant biotechnology journal.2021, 4
[7] Efficient Breeding of Early-Maturing Rice Cultivar by Editing PHYC via CRISPR/Cas9.[J].Li Bin;Du Xi;Fei Yunyan;Wang Fangquan;Xu Yang;LI Xia;Li Wenqi;Chen Zhihui;Fan Fangjun;Wang Jun;Tao Yajun;Jiang Yanjie;Zhu Qian Hao;Yang Jie.Rice.2021,
[8] Transcription factor OsbZIP49 controls tiller angle and plant architecture through the induction of indole-3-acetic acid-amido synthetases in rice
Ding, Chaohui
Lin, Xianhui
Zuo, Ying
Yu, Zhilin
Baerson, Scott R.
Pan, Zhiqiang
Zeng, Rensen
Song, Yuanyuan
[J]. PLANT JOURNAL, 2021, 108 (05): : 1346 - 1364
[9] Identification of Candidate Genes Conferring Cold Tolerance to Rice (Oryza sativa L.) at the Bud-Bursting Stage Using Bulk Segregant Analysis Sequencing and Linkage Mapping.[J].Yang Luomiao;Lei Lei;Li Peng;Wang Jingguo;Wang Chao;Yang Fan;Chen Jiahui;Liu HuaLong;Zheng Hongliang;Xin Wei;Zou Detang.Frontiers in Plant Science.2021,
[10] CRISPR/Cas9-mediated knockout and overexpression studies reveal a role of maize phytochrome C in regulating flowering time and plant height
Li, Quanquan
Wu, Guangxia
Zhao, Yongping
Wang, Baobao
Zhao, Binbin
Kong, Dexin
Wei, Hongbin
Chen, Cuixia
Wang, Haiyang
[J]. PLANT BIOTECHNOLOGY JOURNAL, 2020, 18 (12) : 2520 - 2532

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