Development of the Artificial Antigens for the Organophosphorus Insecticide Chlorpyrifos

被引：0
|
作者：
ZHU Guo-nian1
机构：
来源：
AgriculturalSciencesinChina | 2004年 / 03期
关键词：
Chlorpyrifos; Hapten; Antigen; Synthesis; Identification;
D O I：
暂无
中图分类号：
S482.3 [杀虫剂];
学科分类号：
090403 ;
摘要：
This study reported that the hapten of the organophosphorus insecticide chlorpyrifos, O,O-diethyl-O-[3,5-dichloro-6-(2-carboxyethyl)thio-2-pyridyl]phosphorothioate(named AR) wassynthesized by using technical grade chlorpyrifos reacted with 3-marcapropanoic acid in hotalkaline solution. The hapten was conjugated to bovine serum albumin (BSA) with the modifiedactive ester method to form artificial immune antigen. The ratio of AR:BSA was 39:1. Theartificial coating antigen for chlorpyrifos was synthesized by conjugating AR to ovalbumin(OVA) with the mixed-anhydride method, and the ratio was 13:1. The anti-chlorpyrifos polyclonalantibodies were obtained by using the artificial immune antigen (AR-BSA) to immune in therabbits.
引用
收藏
页码：39 / 45
页数：7
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