Molecular mapping and candidate gene analysis of the semi-dominant gene Vestigial glume1 in maize

被引:1
作者
Chaoxian Liu [1 ]
Yuanzeng Zhao [2 ]
Yang Bai [1 ]
Xiaomin Lu [3 ]
Weibin Song [4 ]
Liping Qin [1 ]
Yilin Cai [1 ]
机构
[1] Maize Research Institute, Southwest University
[2] Henan Institute of Science and Technology
[3] Henan Academy of Agricultural Sciences
[4] National Maize Improvement Center of China, China Agricultural University
关键词
Vestigial glume1; Fine mapping; Candidate gene analysis; ZmCPA-FAS1; Glume development;
D O I
暂无
中图分类号
S513 [玉米(玉蜀黍)]; Q943.2 [植物基因工程];
学科分类号
071007 ; 0901 ; 090102 ;
摘要
The glume is an organ of the maize spikelet and plays important roles in anther and kernel development. Vestigial glume1(Vg1) is a classic mutant associated with ligule and glume development. Here we report the phenotypic characterization, fine mapping, and candidate gene analysis of the Vg1 mutant. Vg1 is a semi-dominant and pleiotropic gene, and also affects plant height, ear height, and tassel length. Vg1 ligule degeneration begins at the first leaf, and the Vg1 tassel and ear can be distinguished from those of wild-type plants when their lengths reach respectively 55 mm and 51 mm. Using a BC3 mapping population of 11,445 plants, we delimited the Vg1 functional site to an interval of 7.4 kb, flanked by the markers In Del LM and CRM6. A putative cyclopropane fatty-acid synthase gene(Zm CPA-FAS1) was hypothesized to underlie the mutant phenotype. We detected a Helitron insertion in the sixth intron of Zm CPAFAS1. Its presence caused abnormal alternative splicing of Zm CPA-FAS1 that conferred new characteristics on the Vg1 mutant. These findings are a basis for further discovery of the molecular mechanism underlying glume development and a potential guide for maize breeding of small-glume varieties, especially sweet corn breeding.
引用
收藏
页码:667 / 676
页数:10
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