Objective: To observe the effect of relaxing needling plus rehabilitation training on post-stroke upper limb dysfunction. Methods: Sixty-eight eligible subjects with post-stoke upper limb dysfunction were randomized into 2 groups by the random number table, 34 in each group, to respectively receive relaxing needling method or ordinary needling method in addition to rehabilitation training for 2 months. Fugl-Meyer assessment of upper limb motor function, neurological defect score, and clinical efficacies were observed and compared. Results: The two groups both showed effects. The total effective rate in the relaxing needling group was 93.8% versus 84.9% in the ordinary needling group, and the difference was statistically significant(P<0.05). The relaxing needling group was better than the ordinary needling group in improving the motor function of upper limb and neurological defect(P<0.05). Conclusion: Relaxing needling plus rehabilitation training can significantly improve the post-stroke motor dysfunction of upper limb, better than ordinary needling plus rehabilitation training.