A bright two-photon fluorescent probe for real-time monitoring autophagy in living cells

被引：0

作者：

Chuankun Jiang ^{[1
]}

Longchun Li ^{[2
]}

Jiacheng Jiang ^{[1
]}

Lilin Hou ^{[1
]}

Gemin Fang ^{[1
,3
]}

Haizhu Yu ^{[1
]}

Xiangming Meng ^{[1
,2
]}

机构：

[1] School of Chemistry and Chemical Engineering,Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials,and Institute of Physical Science and Information Technology,Anhui University

[2] Department of Applied Chemistry,School of science,Anhui Agricultural University

[3] Institute of Physical Science and Information Technology,Anhui University

来源：

Chinese Chemical Letters | 2020年 / 31卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Two-photon; Lysosome-targeting; Polarity; Real-time; Autophagy;

D O I：

暂无

中图分类号：

Q25 [细胞生理学]; O657.3 [光化学分析法（光谱分析法）];

学科分类号：

070302 ; 071009 ; 081704 ; 090102 ;

摘要：

A novel donor-acceptor(D-A) type of two-photon(TP) fluorescent probe,i.e.Lyso-OSC,based on the lysosome-targeting morpholine group was developed.The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor.The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent.The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 μm,respectively.The strong fluorescence,high sensitivity to polarity,low cytotoxicity,and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes ofcellular environment.To this end,a bright,real-time imaging autophagy of living cells has been achieved.

引用

页码：447 / 450

页数：4