miR-29a基于BMP2/Runx2对肋骨骨折大鼠的干预作用

被引：1

作者：

张秀燕 ^{[1
]}

王强 ^{[1
]}

孙晓娟 ^{[1
]}

田乐 ^{[1
]}

高蕾 ^{[1
]}

王海滨 ^{[2
]}

高书军 ^{[1
]}

机构：

[1] 张家口市第二医院手麻科

[2] 张家口市第二医院骨科

来源：

广西医科大学学报 | 2021年 / 38卷 / 11期

关键词：

miR-29; BMP2; RUNX2; 骨折;

D O I：

10.16190/j.cnki.45-1211/r.2021.11.018

中图分类号：

R683.1 [胸骨、肋骨骨折];

学科分类号：

1002 ; 100210 ;

摘要：

目的:探讨miR-29a调控骨形态发生蛋白2(BMP2)/矮小相关转录因子2 (Runx2)在大鼠肋骨骨折愈合中的作用。方法:建立SD大鼠肋骨骨折模型,分为miR-29组和对照组,每组20只。14 d和28 d各处死动物10只,行X线和micro-CT检查,观察骨折愈合情况,分别采用实时荧光定量PCR(RT-qPCR)法和western blotting法检测Runx2和BMP2 mRNA和蛋白表达水平。结果:X线摄片显示自14 d开始两组均有骨痂开始形成,至28 d骨痂塑形改建,骨折愈合。与对照组相比,miR-29组骨密度、骨矿物质含量及骨骼体积比均显著增加,Runx2和BMP2 mRNA和蛋白表达量均明显升高(P<0.05),且28 d时Runx2和BMP2表达量较14 d时明显下降(P<0.05)。结论:miR-29a能够通过Runx2/BMP2信号通路促进骨折部位骨小梁较早的形成和改建,促进矿物质沉积,从而加速骨折愈合。

引用

页码：2128 / 2132

页数：5

共 22 条

[1] BMP2 signalling activation enhances bone metastases of non-small cell lung cancer [J].

Huang, Fei ;

Cao, Yaqiang ;

Wu, Gui ;

Chen, Junying ;

CaihongWang ;

Lin, Wanzun ;

Lan, Ruilong ;

Wu, Bing ;

Xie, Xianhe ;

Hong, Jinsheng ;

Fu, Lengxi .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020, 24 (18) :10768-10784

[2]

Rib fracture displacement worsens over time..[J].Bauman Zachary Mitchel;Grams Benjamin;Yanala Ujwal;Shostrom Valerie;Waibel Brett;Evans Charity Hassie;Cemaj Samuel;Schlitzkus Lisa Lynn.European journal of trauma and emergency surgery : official publication of the European Trauma Society.2020, 6

[3] Molecular Mechanism of Runx2-Dependent Bone Development [J].

Komori, Toshihisa .

MOLECULES AND CELLS, 2020, 43 (02) :168-175

[4] Deficiency of Macf1 in osterix expressing cells decreases bone formation by Bmp2/Smad/Runx2 pathway [J].

Qiu, Wu-Xia ;

Ma, Xiao-Li ;

Lin, Xiao ;

Zhao, Fan ;

Li, Di-Jie ;

Chen, Zhi-Hao ;

Zhang, Ke-Wen ;

Zhang, Ru ;

Wang, Pai ;

Xiao, Yun-Yun ;

Miao, Zhi-Ping ;

Dang, Kai ;

Wu, Xiao-Yang ;

Qian, Ai-Rong .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020, 24 (01) :317-327

[5] MicroRNA-29a represses osteoclast formation and protects against osteoporosis by regulating PCAF-mediated RANKL and CXCL12 [J].

Lian, Wei-Shiung ;

Ko, Jih-Yang ;

Chen, Yu-Shan ;

Ke, Huei-Jing ;

Hsieh, Chin-Kuei ;

Kuo, Chung-Wen ;

Wang, Shao-Yu ;

Huang, Bo-Wun ;

Tseng, Jung-Ge ;

Wang, Feng-Sheng .

CELL DEATH & DISEASE, 2019, 10 (10)

[6] Regulation of microRNA function in animals [J].

Gebert, Luca F. R. ;

MacRae, Ian J. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2019, 20 (01) :21-37

[7] THE BMP PATHWAY AND ITS INHIBITORS IN THE SKELETON [J].

Lowery, Jonathan W. ;

Rosen, Vicki .

PHYSIOLOGICAL REVIEWS, 2018, 98 (04) :2431-2452

[8] Runx2, an inducer of osteoblast and chondrocyte differentiation [J].

Komori, Toshihisa .

HISTOCHEMISTRY AND CELL BIOLOGY, 2018, 149 (04) :313-323

[9]

LncRNA NEAT1/miR-29b-3p/BMP1 axis promotes osteogenic differentiation in human bone marrow-derived mesenchymal stem cells.[J].Yingzi Zhang;Bin Chen;Dazhuang Li;Xiaozhong Zhou;Zhiwei Chen.Pathology - Research and Practice.2018, 3

[10] Deep Sequencing of Human Nuclear and Cytoplasmic Small RNAs Reveals an Unexpectedly Complex Subcellular Distribution of miRNAs and tRNA 3′ Trailers [J].

Liao, Jian-You ;

Ma, Li-Ming ;

Guo, Yan-Hua ;

Zhang, Yu-Chan ;

Zhou, Hui ;

Shao, Peng ;

Chen, Yue-Qin ;

Qu, Liang-Hu .

PLOS ONE, 2010, 5 (05)

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