TWO-SITE PROPERTY OF THENOYLTRIFLUOROACETONE INHIBITING SUCCINATE-UBIQUINONE REDUCTASE

被引：0
|
作者：
徐建兴
TSOO E.KING
机构：
[1] University City Science Center and Department of Biochemistry and Biophysics
[2] Beijing 100080
[3] Philadelphia PA 19104
[4] Academia Sinica
[5] PRC
[6] University of Pennsylvania
[7] Institute for Structural and Functional Studies
[8] Institute of Biophysics
来源：
Science in China,SerB . | 1992年 / Ser.B . 1992查看该刊数据库收录来源卷 / 02期
基金：
中国国家自然科学基金;
关键词：
succinate-ubiquinone reductase; inhibition; TTFA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
It is proved by using the Dixon plot and the Lineweaver-Burk plot that thenoyltrifluoroacetone （TTFA） has two inhibitive sites affecting the reduction of ubiquinone catalyzed by succinate-ubiquinone reductase. The high affinity site （inhibited at the concentration of thenoyltrifluoroacetone less than 20 μmol/L） shows noncompetitive with substrate Q, while the low affinity site （inhibited at the concentration of TTFA over 20 μmol/L） shows competitive. It is suggested that both the reducing steps of Q→QH and QH→QHare inhibited by thenoyltrifluoroacetone.
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页码：162 / 168
页数：7
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