Detection of influenza A virus RNA in birds by optimized Real-Time PCR system

被引：0

作者：

Ilinykh Ph A ^{[1
]}

Shestopalova EM ^{[1
]}

Khripko Yu I ^{[1
]}

Durimanov AG ^{[1
]}

Sharshov KA ^{[1
]}

Shestopalov AM ^{[1
]}

机构：

[1] State Research Center of Virology and Biotechnology Vector,Russia,Novosibirsk region,630559 Koltsovo

来源：

Asian Pacific Journal of Tropical Medicine | 2010年 / 3卷 / 05期

关键词：

Influenza A virus RNA; Real-Time PCR; MDCK cell culture; Virus surveillance; Wild birds;

D O I：

暂无

中图分类号：

S855.3 [病毒病];

学科分类号：

0906 ;

摘要：

Objective:To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds.Methods:Sensitivity,specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed.Results: Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell culture.Conclusions:Real-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.

引用

页码：337 / 340

页数：4