The dwarf vine and small fruit watermelon, referred to as ‘dsh’ in this study, is a natural mutant of the ordinary watermelon, ‘Hanxuan Lvyuan’.Morphological assessment indicated that its main vine length, internode length, and single-fruit weight were significantly lower than those of the ordinary watermelon(P < 0.05). After crossing the mutant with two ordinary watermelons, following by inbreeding the F;and backcrossing(BC)F;to ‘dsh’, the F;, F;, and BC generations were obtained. Genetic analysis of vine type and single-fruit weight showed that the F;generation consisted of long-vine plants, with single-fruit weight significantly higher than that of the ordinary parent watermelon(P < 0.05). The long and short vine plant ratios of the F;and BC populations conform to the Mendel’s segregation ratios of 3:1 and 1:1(P > 0.05), respectively. It was suggested that this dwarf and small fruit mutation was a single recessive gene trait that followed a Mendel’s inheritance.