Interplay of neuropilin-1 and semaphorin 3A after partial hepatectomy in rats

被引:0
作者
Tsuneo Kitamura [1 ,2 ,3 ]
Kazuhisa Iwabuchi [1 ]
Syozo Ichinose [1 ]
Mitsuaki Yanagida [1 ]
Hideoki Ogawa [1 ]
Sumio Watanabe [3 ]
Toshihide Maruyama [2 ,3 ]
Masafumi Suyama [2 ,3 ]
Kenji Takamori [1 ]
机构
[1] Institute for Environmental and Gender Specific Medicine,Juntendo University Graduate School of Medicine,Urayasu 279-0021,Japan
[2] Department of Gastroenterology,Juntendo University Urayasu Hospital,Urayasu 279-0021,Japan
[3] Department of Gastroenterology,Juntendo University School of Medicine,Tokyo 113-8421,Japan
基金
日本学术振兴会;
关键词
Neuropilin-1; Semaphorin; 3A; Sinusoidal remodeling; Liver regeneration; Two-thirds partial hepatectomy; Angiogenesis; Liver sinusoidal endothelial cells;
D O I
暂无
中图分类号
R657.3 [肝及肝管];
学科分类号
1002 ; 100210 ;
摘要
AIM:To elucidate the role of neuropilin-1(Nrp-1) and semaphorin 3A(Sema3A) in sinusoidal remodeling during liver regeneration in rats.METHODS:Male Wistar/ST rats at 7 wk of age,weighing about 200 g,were used for all animal experiments.In vivo,at 24,48,72,96,144 and 192 h after twothirds partial hepatectomy(PHx),the remnant livers were removed.Liver tissues were immunohistochemically stained for Nrp-1,Sema3A and SE-1,a liver sinusoidal endothelial cell(SEC) marker.Total RNA of the liver tissue was extracted and reversely transcribed into cDNA.The mRNA expression of Sema3A was analyzed by quantitative real-time polymerase chain reaction and normalized to that of ribosomal protein S18.In vitro,SECs were isolated from rat liver and cultured in endothelial growth medium containing 20 ng/mL vascular endothelial cell growth factor.Migration of SECs in primary culture was assessed by cell transwell assay with or without recombinant Sema3A.Apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method.RESULTS:In vitro,immunohistochemistry study revealed that Sema3A and Nrp-1 were constitutively expressed in hepatocytes and SECs,respectively,in normal rat liver tissues.Nrp-1 expression in SECs was quantified by the percentage of immunostained area with antiNrp-1 antibody in relation to the area stained with SE-1.Between 24 h and 96 h following resection of liver,Nrp-1 expression in SECs was transiently increased.Compared with the baseline(5.2% ± 0.1%),Nrp-1 expression in SECs significantly increased at 24 h(17.3% ± 0.7%,P < 0.05),48 h(39.1% ± 0.6%,P < 0.01),72 h(46.9% ± 4.5%,P < 0.01) and 96 h(29.9% ± 3.8%,P < 0.01) after PHx,then returned to the basal level at termination of liver regeneration.Interestingly,the expression of Sema3A was inversely associated with that of Nrp-1 in liver after PHx.Sema3A mRNA expression was significantly reduced by about 75% over the period 24-144 h after PHx(P < 0.05),and returned to basal levels at 192 h after PHx.In vitro,SECs isolated from rats after PHx(PHx-SECs) were observed to migrate to the lower chamber of the cell transwell system after incubation for 24 h,but not cells from normal rats(CONT-SECs),indicating that mobility of PHx-SECs increases as compared with that of CONT-SECs.Moreover,recombinant Sema3A significantly attenuated migration in PHx-SECs in primary culture(vehicle-treated 100% ± 7.9% vs Sema3A-treated 42.6% ± 5.4%,P < 0.01),but not in CONT-SECs.Compared with CONTSECs,the apoptotic rate of PHx-SECs decreased by 78.3%(P < 0.05).There was no difference in apoptosis between CONT-SECs that were treated with vehicle and Sema3A.However,in PHx-SECs,apoptosis was induced by the presence of 5 nmol Sema3A for 24 h(vehicle-treated 21.7% ± 7.6% vs Sema3A-treated 104.3% ± 8.9%,P < 0.05).In addition,immunohistochemistry confirmed the increased expression of Nrp-1 in PHx-SECs,while it was noted to a lesser extent in CONT-SECs.CONCLUSION:The interplay of Nrp-1 and Sema3A shown in our results may lead to a better understanding of interaction between sinusoidal remodeling and SECs during liver regeneration.
引用
收藏
页码:5034 / 5041
页数:8
相关论文
共 50 条
[31]   Semaphorin-3A and its receptor neuropilin-1 are predominantly expressed in endothelial cells along the rostral migratory stream of young and adult mice [J].
Melendez-Herrera, Esperanza ;
Colin-Castelan, Dannia ;
Varela-Echavarria, Alfredo ;
Gutierrez-Ospina, Gabriel .
CELL AND TISSUE RESEARCH, 2008, 333 (02) :175-184
[32]   Higher mortality in heterozygous neuropilin-1 mice after cardiac pressure overload [J].
Li, Fei ;
Zhao, Hui ;
Liao, Yulin ;
Takashima, Seiji ;
Asano, Yoshihiro ;
Shintani, Yasunori ;
Hori, Masatsugu ;
Kitakaze, Masafumi .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 370 (02) :317-321
[33]   The effect of sorafenib on liver regeneration and angiogenesis after partial hepatectomy in rats [J].
Kiroplastis, K. ;
Fouzas, I ;
Katsiki, E. ;
Patsiaoura, K. ;
Daoudaki, M. ;
Komninou, A. ;
Xolongitas, E. ;
Katsika, E. ;
Kaidoglou, K. ;
Papanikolaou, V .
HIPPOKRATIA, 2015, 19 (03) :249-255
[34]   Transcriptome analysis of hepatocytes after partial hepatectomy in rats [J].
Xu, Cunshuan ;
Chen, Xiaoguang ;
Chang, Cuifang ;
Wang, Gaiping ;
Wang, Wenbo ;
Zhang, Lianxing ;
Zhu, Qiushi ;
Wang, Lei ;
Zhang, Fuchun .
DEVELOPMENT GENES AND EVOLUTION, 2010, 220 (9-10) :263-274
[35]   Semaphorin3A-neuropilin1 signalling is involved in the generation of cortical interneurons [J].
Andrews, William D. ;
Barber, Melissa ;
Nemitz, Marion ;
Memi, Fani ;
Parnavelas, John G. .
BRAIN STRUCTURE & FUNCTION, 2017, 222 (05) :2217-2233
[36]   Semaphorin 3a transfection into the left stellate ganglion reduces susceptibility to ventricular arrhythmias after myocardial infarction in rats [J].
Yang, Ling-Chao ;
Zhang, Peng-Pai ;
Chen, Xiao-Meng ;
Li, Chang-Yi ;
Sun, Jian ;
Hou, Jian-Wen ;
Chen, Ren-Hua ;
Wang, Yue-Peng ;
Li, Yi-Gang .
EUROPACE, 2016, 18 (12) :1886-1896
[37]   An Unexpected Role of Semaphorin3A-Neuropilin-1 Signaling in Lymphatic Vessel Maturation and Valve Formation [J].
Jurisic, Giorgia ;
Maby-El Hajjami, Helene ;
Karaman, Sinem ;
Ochsenbein, Alexandra M. ;
Alitalo, Annamari ;
Siddiqui, Shoib S. ;
Pereira, Carlos Ochoa ;
Petrova, Tatiana V. ;
Detmar, Michael .
CIRCULATION RESEARCH, 2012, 111 (04) :426-U196
[38]   Expression of Semaphorin 4A and Neuropilin-1 in Peripheral Blood of Patients with Rheumatoid Arthritis and Their Relationship with Regulatory T Cell/Helper T Cells 17 Balance [J].
Zhou, Yu ;
Yang, Xiaoqiong ;
Chen, Zhijian .
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2021, 83 :1-5
[39]   Glycan-dependent binding of galectin-1 to neuropilin-1 promotes axonal regeneration after spinal cord injury [J].
Quinta, H. R. ;
Pasquini, J. M. ;
Rabinovich, G. A. ;
Pasquini, L. A. .
CELL DEATH AND DIFFERENTIATION, 2014, 21 (06) :941-955
[40]   Semaphorin 3A及其受体Neuropilin-1在人舌鳞状细胞癌/大鼠背根神经节共培养中对轴突芽生的影响 [J].
唐子春 ;
王子露 ;
宋晓萌 ;
朱江 ;
吴煜农 .
口腔生物医学, 2012, 3 (04) :189-192+200