Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy

被引：3

作者：

Bin Liu ^{[1
,2
]}

Wen Cao ^{[3
]}

Jin Cheng ^{[1
,2
]}

Sisi Fan ^{[1
,2
]}

Shaojun Pan ^{[4
]}

Lirui Wang ^{[1
,2
]}

Jiaqi Niu ^{[1
,2
]}

Yunxiang Pan ^{[1
,2
]}

Yanlei Liu ^{[1
,2
]}

Xiyang Sun ^{[5
]}

Lijun Ma ^{[5
]}

Jie Song ^{[1
,2
]}

Jian Ni ^{[1
,2
]}

Daxiang Cui ^{[1
,2
,5
]}

机构：

[1] Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Centre for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai

[2] National Center for Translational Medicine,Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University

[3] Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University

[4] School of Biomedical Engineering, Shanghai Jiao Tong University

[5] Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

来源：

Cancer Biology & Medicine | 2019年 / 16卷 / 04期

关键词：

Gold nanostars; natural killer cells; photothermal therapy; photodynamic therapy; immunotherapy;

D O I：

暂无

中图分类号：

R730.5 [肿瘤治疗学];

学科分类号：

100214 ;

摘要：

Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars（GNSs） with Chlorin e6 molecules（Ce6） into human peripheral blood mononuclear cells（PBMCs）-derived NK cells for tumor targeted therapy.Methods:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and UV-vis.The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry（FCM） and ELISA.Effects of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and CCK-8.Intracellular fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic（CLSM） and FCM at different time points.Intracellular ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by FCM.The distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA imaging.The combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing mice.Results:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake process.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic imaging.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and immunotherapy.Conclusions:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing mice.Through fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future.

引用

页码：756 / 770

页数：15

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