Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization

被引：0
作者：
Li Ming DU1*
机构：
来源：
ChineseChemicalLetters | 2001年 / 11期
关键词：
Sparfloxacin; HPLC; pre-column derivatization; human urine;
D O I：
暂无
中图分类号：
O657.7 [色谱分析];
学科分类号：
070302 ; 081704 ;
摘要：
Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%～95.7% and the RSD is 1.2%~4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.
引用
收藏
页码：1007 / 1010
页数：4
相关论文
共 50 条
  • [11] HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY DETERMINATION OF ANILINES WITH FLUORESCENT DETECTION AND PRE-COLUMN DERIVATIZATION
    Al-Kindy, Salma M. Z.
    Al-Kalbani, Azza
    Al-Harasi, Ahmed F.
    Suliman, FakhrEldin O.
    Al-Lawati, Haider J.
    Al-Hamadi, Abdalla
    INSTRUMENTATION SCIENCE & TECHNOLOGY, 2013, 41 (01) : 48 - 59
  • [12] Determination of trace amounts of bismuth in iron and steel by pre-column derivatization reversed-phase high-performance liquid chrorriatography with ethylenediaminetetraacetate
    Muramoto, Taihei
    Yukishita, Yoshitaka
    Shimizu, Tokuo
    Uehara, Nobuo
    TETSU TO HAGANE-JOURNAL OF THE IRON AND STEEL INSTITUTE OF JAPAN, 2007, 93 (02): : 94 - 99
  • [13] Determination of biogenic amines in beer with pre-column derivatization by high performance liquid chromatography
    Tang, Tao
    Shi, Tianyu
    Qian, Kun
    Li, Pingliang
    Li, Jianqiang
    Cao, Yongsong
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (5-6): : 507 - 512
  • [14] Determination of histamine in canned fish by high performance liquid chromatography with pre-column derivatization
    Jin Gaowa
    Cai Youqiong
    Yu Huijuan
    Qian Beilei
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2010, 28 (11) : 1099 - 1102
  • [15] Fluorometric determination of pantothenic acid in human urine by isocratic reversed-phase ion-pair high-performance liquid chromatography with post-column derivatization
    Takahashi, Kei
    Fukuwatari, Tsutomu
    Shibata, Katsumi
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (22): : 2168 - 2172
  • [16] Determination of free amino acids in the pork and its broth cooked by different methods using reversed-phase high performance liquid chromatography coupled with pre-column derivatization
    Gu Weigang
    Zhang Jinjie
    Xin Mei
    Yao Yanjia
    Ji Rong
    Lu Bingbing
    Chen Jianchu
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2011, 29 (10) : 1041 - 1045
  • [17] Determination of 1-deoxynojirimycin in Morus alba L. leaves using reversed-phase high performance liquid chromatography fluorescence detection with pre-column derivatization
    Xie Huiming
    Wu Fangrui
    Yang Yi
    Liu Jie
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2008, 26 (05) : 634 - 636
  • [18] Determination of kanamycin A in animal feeds by solid phase extraction and high performance liquid chromatography with pre-column derivatization and fluorescence detection
    Zhou, Y. X.
    Yang, W. J.
    Zhang, L. Y.
    Wang, Z. Y.
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2007, 30 (9-12) : 1603 - 1615
  • [19] Determination of free formaldehyde in cosmetics by pre-column derivatization, extraction inhibition and high performance liquid chromatography
    Lu Chunhua
    Huang Chaoqun
    Chen Mei
    Xie Wen
    Chen Xiaomei
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2012, 30 (12) : 1287 - 1291
  • [20] Determination of optical purity of alpha-phenylethylamine by high performance liquid chromatography with pre-column derivatization
    Wang Jinzhao
    Zeng Su
    Wang Danhua
    Hu Gongyun
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2009, 27 (03) : 376 - 378
12345