Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization

被引：0

作者：

Li Ming DU Zhe Feng FAN Jin Li QIAO Jing Ping WANG Analysis and Test Center Shanxi Teachers University Lingfen Department of Chemistry Arts and Sciences College Taiyuan University of Technology Taiyuan ^{[1
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机构：

来源：

Chinese Chemical Letters | 2001年 / 11期

关键词：

Sparfloxacin; HPLC; pre-column derivatization; human urine;

D O I：

暂无

中图分类号：

O657.7 [色谱分析];

学科分类号：

070302 ; 081704 ;

摘要：

Sparfloxacin can be oxidized by nitrous acid, then react with hydroiodic acid to form a fluorescent derivative. Based on this, a reversed-phase high performance liquid chromatographic pre-column derivatization new method is described for the determination of sparfloxacin in human urine. The linear range is 0.05 mg/L to 4.0 mg/L, the recoveries are 91.5%～95.7% and the RSD is 1.2%4.2%. The results showed that this method is suitable for the determination of sparfloxacin in human urine.

引用

页码：1007 / 1010

页数：4