Sequence-specific Hydrolysis of Single-stranded DNA by PNA-Cerium (IV) Adduct

被引：0
|
作者：
He Bai SHEN
机构：
来源：
Chinese Chemical Letters | 2005年 / 12期
关键词：
Peptide nucleic acid (PNA); artificial site specific cleavage reagent; hydrolysis; reversed-phase high-performance liquid chromatogram (RP-HPLC);
D O I：
暂无
中图分类号：
Q523 [脱氧核糖核酸（DNA）];
学科分类号：
摘要：
A novel artificial site specific cleavage reagent, with peptide nucleic acid (PNA) as sequence-recognizing moiety and cerium (IV) ions as “scissors” for cleaving target DNA, was synthesized. Subsequently, it was employed in the cleavage of target 26-mer single-stranded DNA (ssDNA), which has 10-mer sequence complementary with PNA recognizer in the hybrids, under physiological conditions. Reversed-phase high-performance liquid chromatogram (RP- HPLC) experiments indicated that the artificial site specific cleavage reagent could cleave the target DNA specifically.
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页码：1663 / 1666
页数：4
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