Expression analysis of gdcsP promoter from C3-C4 intermediate plant Flaveria anomala in transgenic rice

The gdcsP promoter isolated from C3-C4 intermediate plant Flaveria anomala was fused to the β-glu-curonidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced into the rice (Oryza saliva L.) cv. 8706 by Agrobacterium-mediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However, no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression declined significantly with the maturity of plants. These expression patterns make the gdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues.