Cloning, Sequencing and Expression in E. coli of Interferon-ω1 Gene

Human interferon ω1 (huIFN-ω1) gene was isolated and cloned from chromosome DNA derived from a Chinese fetal liver via polymerase chain reaction (PCR). By determining its nucleotide sequence we proved that the 88th codon should be GGA, coding for Gly. After engineering the original IFN-ω1 gene clone to a form that may be expressed as a nonfused protein, we also took the IFN-ω1 gene under the control of the PRPL promoter with an expression vector pBV220 in E. coli. The antivirus activity of the recombinant IFN-ω1 is about 6.5×10~7 units/L CULTURE (OD600=0.75). Since IFN-ω1 not only has antivirus activity but also shows considerably high homology with animal trophoblast proteins which have been proved antiluteolysins as a maternal recognition signal for pregnancy, we believe that study on it will be practically and theoretically significant.