The Determination of Canthaxanthin in Feed Ingredients by High Performance Liquid Chromatography

A series of studies were conducted to establish a methodology for the accurate and efficient determination of can-thaxanthin in feed ingredients.Agilent ZORBAX Eclipse DB-C18 analytical column(4.6×150 mm,5 μm) was used and kept at 25℃.The mobile phase was acetonitrile:methanol(v/v)=95 :5,and eluted compounds were detected by DAD absorbance(470 nm).The flow rate was maintained at 1.0 mL · min-1.The response of the method was linear over the range 1.0-20.0 μg· mL-1 canthaxanthin assay solution(R2=0.9998).Recovery assays done with standard canthaxanthin to feed ingredient resulted in an average recovery of 103%.Variation coefficient was less than 3.53%.This method is proved to be simple,precise,sensitive and reproductive.