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Effects of ginkgo biloba extract on the expressions of IL-1β,TNF-α,IL-10 and IL-10R in heart of atherosclerotic rats
被引:1
作者:
焦亚斌
芮耀诚
李铁军
杨鹏远
邱彦
机构:
[1] China
[2] Shanghai 200433
[3] Department of Pharmacology School of Pharmacy Second Military Medical University
基金:
中国国家自然科学基金;
关键词:
atherosclerosis;
cytokine;
interleukin-1;
tumor necrosis factor;
interleukin-10;
Ginkgo biloba;
rats;
D O I:
暂无
中图分类号:
R543 [血管疾病];
学科分类号:
1002 ;
100201 ;
摘要:
Objective: To study whether the anti-AS effect of ginkgo biloba extract (GbE) was related with inhibitory effects on the expression of IL-1β, TNF-αand up-regulation of IL-10 and IL-10R in the heart of atherosclerotic (AS) rats. Methods : The experimental model of AS rats were established by in-traperitioneal injection of vitamin D3 with high fat and cholesterol diet. All rats were divided into 3 groups: control,AS and GbE. GbE (100 mg/kg) was administered to rats by ig. After 8 weeks, the expression of IL-1β, TNF-α, IL-10, and IL-10R in the heart of AS rats were detected by enzyme-linked immunosorbant assay, reverse transcriptase polymerasechain reaction and Western blotting. Results:The protein and mR-NA expressions of IL-1β,TNF-α,IL-10 and IL-10R were markedly higher in AS group than those in control group (P<0. 01). The protein and mRNA expressions of IL-1βand TNF-αwere markedly lower in GbE group than those in AS group; while the protein and mRNA expressions of IL-10 and IL-10R were markedly higher in GbE group than those in AS group (P<0. 01). Conclusion: GbE has significant inhibitory effects on proinflammatory cytokine IL-1β, TNF-α. The up-regulation of anti-inflammatory cy-tokine IL-10,IL-10R that may be partially responsible for its anti-AS effects.
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页码:347 / 351
页数:5
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