A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase

被引：0

作者：

Wei Shi ^{[1
,2
]}

Peng Gong ^{[1
]}

机构：

[1] Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences

[2] University of Chinese Academy of Sciences

来源：

Virologica Sinica | 2017年 / 04期

基金：

中国国家自然科学基金;

关键词：

HDV; A practical approach to generate suitable de novo synthesis RNA template for a flavivirus RNA-dependent RNA polymerase; RNA;

D O I：

暂无

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

Dear Editor,Due to its high transcription efficiency,bacteriophage T7RNA polymerase(T7 RNAP)has long been utilized in bacterial and in vitro systems to generate large quantity of RNA for various purposes(Studier and Moffatt,1986).However,heterogeneity at the 3′-end of the RNA transcript remains a major limitation for in vitro RNA

引用

页码：331 / 334

页数：4

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