基质硬度对2D和3D培养乳腺癌细胞摄取纳米制剂行为的影响

被引:3
作者
王瑜
秦硕
龚涛
张志荣
符垚
机构
[1] 四川大学华西药学院靶向药物与释药系统教育部重点实验室
关键词
基质硬度; 二维/三维细胞培养; 细胞形态; 细胞摄取; 聚苯乙烯纳米球;
D O I
10.16438/j.0513-4870.2017-0326
中图分类号
R943 [制剂学];
学科分类号
100602 ; 100702 ;
摘要
以聚苯乙烯纳米球为研究对象,研究基质硬度对二维(2D)和三维(3D)培养的乳腺癌细胞摄取行为的影响。通过光催化聚合反应法制备硬度可调的聚丙烯酰胺凝胶,并在凝胶表面共价修饰胶原以支持细胞黏附,构建2D细胞培养模型;选择Ⅰ型鼠尾胶原构建3D细胞培养模型;分别接种人乳腺癌细胞MCF-7于聚丙烯酰胺凝胶表面或混悬于胶原凝胶中进行3D细胞培养;20 nm和50 nm聚苯乙烯纳米球悬液与2D或3D培养的细胞共孵育,采用激光共聚焦显微镜对细胞形态和纳米球摄取效率进行定性和定量考察。本研究发现在2D和3D培养条件下,基质硬度均参与调控肿瘤细胞肌动蛋白骨架的重构并间接影响不同粒径大小的纳米制剂的摄取行为。
引用
收藏
页码:1324 / 1330
页数:7
相关论文
共 14 条
[1]  
3D scaffolds in breast cancer research[J] . Girdhari Rijal,Weimin Li.Biomaterials . 2016
[2]  
“Combo” nanomedicine: Co-delivery of multi-modal therapeutics for efficient, targeted, and safe cancer therapy[J] . Jessica A. Kemp,Min Sun Shim,Chan Yeong Heo,Young Jik Kwon.Advanced Drug Delivery Reviews . 2015
[3]  
A 3D tension bioreactor platform to study the interplay between ECM stiffness and tumor phenotype[J] . Luke Cassereau,Yekaterina A. Miroshnikova,Guanqing Ou,Johnathon Lakins,Valerie M. Weaver.Journal of Biotechnology . 2015
[4]  
Three‐dimensional models of cancer for pharmacology and cancer cell biology: Capturing tumor complexity in vitro/ex vivo[J] . John A. Hickman,Ralph Graeser,Ronald de Hoogt,Suzana Vidic,Catarina Brito,Matthias Gutekunst,Heiko van der Kuip,IMI PREDECT consortium.Biotechnology Journal . 2014 (9)
[5]  
Stimuli-responsive cross-linked micelles for on-demand drug delivery against cancers[J] . Yuanpei Li,Kai Xiao,Wei Zhu,Wenbin Deng,Kit S. Lam.Advanced Drug Delivery Reviews . 2014
[6]  
Evaluation of normal and metastatic mammary cells grown in different biomaterial matrices: establishing potential tissue test systems[J] . BrianW. Booth,Jang Pyo Park,KarenJ.L. Burg.Journal of Biomaterials Science, Polymer Edition . 2013 (6)
[7]  
The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs[J] . Lei Chen,Zhifeng Xiao,Yue Meng,Yannan Zhao,Jin Han,Guannan Su,Bing Chen,Jianwu Dai.Biomaterials . 2011 (5)
[8]  
Substrate stiffness regulates apoptosis and the mRNA expression of extracellular matrix regulatory genes in the rat annular cells[J] . Yue-Hui Zhang,Chang-Qing Zhao,Lei-Sheng Jiang,Li-Yang Dai.Matrix Biology . 2010 (2)
[9]   Influence of Substrate Stiffness on the Phenotype of Heart Cells [J].
Bhana, Bashir ;
Iyer, Rohin K. ;
Chen, Wen Li Kelly ;
Zhao, Ruogang ;
Sider, Krista L. ;
Likhitpanichkul, Morakot ;
Simmons, Craig A. ;
Radisic, Milica .
BIOTECHNOLOGY AND BIOENGINEERING, 2010, 105 (06) :1148-1160
[10]  
Cellular Uptake of Fluorescent Labelled Biotin–Streptavidin Microspheres[J] . M. Bradley,L. Alexander,R. M. Sanchez-Martin.Journal of Fluorescence . 2008 (3-4)