Electrochemical Determination of Alkaline Phosphatase in Human Serum by Differential Pulse Voltammetry

被引：0

作者：

SUN Wei JIAO Kui WANG Haiyu and LU Lude College of Chemical Engineering Nanjing University of Science and Technology Nanjing P R China College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao P R China ^{[1
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机构：

来源：

Chemical Research in Chinese Universities | 2004年 / 06期

关键词：

Alkaline phosphatase; Phenyl phosphate; Enzymatic analysis; Differential pulse voltammetry; Human serum sample;

D O I：

暂无

中图分类号：

O657.1 [电化学分析法];

学科分类号：

070302 ; 081704 ;

摘要：

Differential pulse voltammetry（DPV） was applied to the determination of alkaline phosphatase（ALP） activity in human serum with phenyl phosphate as the substrate. Phenyl phosphate can enzymatically be hydrolyzed to produce phenol which is quantified by DPV at +660 mV（vs.Ag/AgCl） in the concentration range of 2.0100 μmol/L. The standard curve for ALP is linear over the range from 0.06 to 1000 U/L with a relative standard deviation of 3.0%. The conditions for the enzymatic reaction and voltammetric detection were optimized and the kinetic constants were also examined.The human serum samples were tested by this method and the results were in good agreement with those obtained by the routine p-nitrophenyl phosphate spectrophotometric method.

引用

页码：690 / 693

页数：4

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