Effect of PKC pathway on G1/S progression control in HeLa cells

The effect of PKC activity on G1/S progression in HeLa cells has been studied. The result shows that (i) PKC activity alteration in G1 phase affects d/S progression in HeLa cells. it has been observed that G1/S progression is stimulated by PKC agonist TPA and inhibited by PKC inhibitor GF-109203X. (ii) The expression of c-myc and c-jun is stimulated by TPA and inhibited by GF-109203X treatment in early G1 phase. (iii) During G1/S progression, the expression of CyciinD1is stimulated by TPA treatment and inhibited by GF-109203X treatment. There is no effect on the expression of CDK4. it is likely that PKC pathway regulates G1/S progression through regulating the expression of some early response genes and engine molecules in HeLa cells.