The Effect of Lumbrokinase on P-selectin and E-selectin in Cerebral Ischemia Model of Rat

Purpose:To find the effect of lumbrokinase(LK)on P-selectin and E-selectin in ischemic rats.Methods:Male healthy Spragur-Dawley rats weighing 180-220 g(n=90) were divided into 4 groups:(1)normalcontrol group(n=5),(2)sham-operated group(n=35),(3)ischemic group(n=35),(4)LK group(n=15).LK10mg/kg(2000UK activity of LK)was given by intraperitoneal injection in the LK group 30 minutes beforeexperiment.Same volume of normal saline was given in the sham-operated group and ischemic group.Theischemic model was made by modified Haruo Nagasawa’s method.Immunohistochemistry was used toobserve the P-selectin and E-selectin positive cells in the ischemic region.Results:P-selectin and E-selectinpositive cells in ischemic regions were observed in the ischemic group,and the peak of expression was at 6hours and 12 hours,respectively.The similar changes were not observed in normal control group.Therewere only a few positive cells in the sham-operated group.In LK group,the P-selectin and E-selectinpositive cells were significantly less than those in the ischemic group(P<0.05 at 3 hours after the onset,P<0.01 at 6 hours and P<0.01 at 12 hours,respectively).Conclusions:LK might significantly decrease theimmunoreactions of P-selectin and E-selectin in ischemic lesion.