SOX4能与p53蛋白发生相互作用

被引:1
|
作者
潘欣
何昆
张维娜
李慧艳
赵洁
周涛
张学敏
李爱玲
机构
[1] 国家生物医学分析中心
关键词
SOX4; p53; 相互作用;
D O I
暂无
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
目的:研究SOX4和p53蛋白之间的相互作用。方法:应用GSTpull-down、免疫共沉淀实验验证相互作用。结果:GSTpulldown实验证实SOX4能结合GST-p53融合蛋白,但不能结合GST蛋白;免疫共沉淀实验也证明,SOX4与p53能在细胞内发生相互作用。结论:SOX4能与p53发生相互作用,为p53信号通路的研究提供了新的线索。
引用
收藏
页码:817 / 819
页数:3
相关论文
共 16 条
  • [1] SOX4 expression in bladder carcinoma: clinical aspects and in vitro functional characterization. Aaboe M,Birkenkamp-Demtroder K,Wiuf C,et al. Cancer Research . 2006
  • [2] Levine AJ Surfing the p53 network. Vogelstein B,Lane D. Nature . 2000
  • [3] Analysis of the molecular cascade responsible for mesodermal limb chondrogenesis: Sox genes and BMP signaling. Chimal-Monroy J,,Rodriguez-Leon J,Montero JA, et al. Developmental Biology . 2003
  • [4] Normal lung development and function after Sox9 inactivation in the respiratory epithelium. Perl AK,Kist R,Shan Z,et al. Genesis . 2005
  • [5] Wild-type p53:tumors can‘t stand it. Kastan MB. Cell . 2007
  • [6] Regulation and function of the p53 tumor suppressor protein. Ryan KM,Phillips AC,Vousden KH. Current Opinion in Cell Biology . 2001
  • [7] Defects in car-diac outflow tract formation and pro-B-lymphocyte expansion in mice lacking Sox-4. Schilham M W,,Oosterwegel M A,Moerer P,et al. Nature . 2006
  • [8] Sox-4facilitates thymocyte differentiation. Schilham MW,Moerer P,Cumano A,et al. European Journal of Immunology . 1997
  • [9] Knockdown of Sox4expression by RNAi induces apoptosis in ACC3cells. Pramoonjago P,Baras AS,Moskaluk CA. Oncegene . 2006
  • [10] Transcriptional control of early B cell development. Busslinger M. Annual Review of Immunology . 2004