Domain Ⅲ of Dengue Virus Serotype 2 Envelope: Expression at High Levels in Escherichia coli and Competitive Inhibition of Virus Entry

被引：0
作者：
Yong-hong Yang [1 ]
Xue-li Zheng [1 ]
Lei-zi Qing [1 ]
Pin-ting Zhu [1 ]
Jing Pan [1 ]
Lei Luo [2 ]
机构：
[1] Department of Etiobiology of School of Public Health and Tropical Medicine of South Medical University
[2] Guangzhou Center for Disease Control and Prevention,No.1, Qide Road of Baiyun District
来源：
Infection International(Electronic Edition) | 2013年 / 2卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Dengue virus; Envelope domain Ⅲ; Virus entry; Vaccine; Neutralizing antibodies;
D O I：
暂无
中图分类号：
R373 [人体病毒学（致病病毒）];
学科分类号：
100103 ; 100705 ;
摘要：
Obejective The domainⅢof dengue virus type 2 envelope was cloned and expressed in Escherichia coli and the inhibited effects of recombinant protein on virus was detected.Methods In this study,the domainⅢ(DⅢ)protein of the dengue virus type-2(DENV-2)envelope(E)antigen was expressed in Escherichia coli by fusion with a carrier protein.The protein was purified using enzymatic cleavage and affinity purification.Rabbit immunization and antibody detection was carried out.Inhibition of DENV-2 infection was observed by DENV-2 EDⅢprotein and its immunity rabbits serum.Results The recombinant expression DENV-2 EDⅢprotein plasmid was constructed successfully.After isopropyl thiogalactoside induction,a specific soluble 29 kD protein was obtained,and the expression product
引用
收藏
页码：114 / 122
页数：9
相关论文
共 50 条
  • [31] The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection
    Liu, Yanxia
    Zhou, Yanhe
    Wu, Jinya
    Zheng, Peiming
    Li, Yiji
    Zheng, Xiaoying
    Puthiyakunnon, Santhosh
    Tu, Zhijian
    Chen, Xiao-Guang
    CELL AND BIOSCIENCE, 2015, 5
  • [32] Pichia pastoris-expressed dengue virus type 2 envelope domain III elicits virus-neutralizing antibodies
    Batra, Gaurav
    Raut, Rajendra
    Dahiya, Satinder
    Kamran, Neha
    Swaminathan, Sathyamangalam
    Khanna, Navin
    JOURNAL OF VIROLOGICAL METHODS, 2010, 167 (01) : 10 - 16
  • [33] The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection
    Yanxia Liu
    Yanhe Zhou
    Jinya Wu
    Peiming Zheng
    Yiji Li
    Xiaoying Zheng
    Santhosh Puthiyakunnon
    Zhijian Tu
    Xiao-Guang Chen
    Cell & Bioscience, 5
  • [34] Serotype specificity of recombinant fusion protein containing domain III and capsid protein of dengue virus 2
    Izquierdo, Alienys
    Valdes, Iris
    Gil, Lazaro
    Hermida, Lisset
    Gutierrez, Sheila
    Garcia, Angelica
    Bernardo, Lidice
    Pavon, Alekis
    Guillen, Gerardo
    Guzman, Maria G.
    ANTIVIRAL RESEARCH, 2012, 95 (01) : 1 - 8
  • [35] Expression and assembly of cholera toxin B subunit and domain III of dengue virus 2 envelope fusion protein in transgenic potatoes
    Kim, Mi-Young
    Li, Jing-Ying
    Nguyen-Quang-Duc Tien
    Yang, Moon-Sik
    PROTEIN EXPRESSION AND PURIFICATION, 2017, 139 : 57 - 62
  • [36] A consensus envelope protein domain III can induce neutralizing antibody responses against serotype 2 of dengue virus in non-human primates
    Hsin-Wei Chen
    Shih-Jen Liu
    Yi-Shiuan Li
    Hsueh-Hung Liu
    Jy-Ping Tsai
    Chen-Yi Chiang
    Mei-Yu Chen
    Chyi-Sing Hwang
    Chin-Cheng Huang
    Hui-Mei Hu
    Han-Hsuan Chung
    Sze-Hsien Wu
    Pele Chong
    Chih-Hsiang Leng
    Chien-Hsiung Pan
    Archives of Virology, 2013, 158 : 1523 - 1531
  • [37] Adenovirus-Mediated Expression of Dengue Virus 2 Envelope Ferritin Nanoparticles Induced Virus-Specific Immune Responses in BALB/c Mice
    Tennakoon, M. S. B. W. T. M. Nipuna Sudaraka
    Ryu, Ji-Hoon
    Jung, Yong-Sam
    Qian, Yingjuan
    Shin, Hyun-Jin
    MICROBIOLOGY RESEARCH, 2024, 15 (03) : 1913 - 1923
  • [38] Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems
    Gottschamel, Johanna
    Loessl, Andreas
    Ruf, Stephanie
    Wang, Yanliang
    Skaugen, Morten
    Bock, Ralph
    Clarke, Jihong Liu
    PLANT MOLECULAR BIOLOGY, 2016, 91 (4-5) : 497 - 512
  • [39] Effect of the Rho GTPase inhibitor-1 on the entry of dengue serotype 2 virus into EAhy926 cells
    Dongying Fan
    Na Wu
    Jing Zhang
    Zhaoyang Wang
    Peigang Wang
    Na Gao
    Jing An
    Molecular Biology Reports, 2020, 47 : 9739 - 9747
  • [40] Effect of the Rho GTPase inhibitor-1 on the entry of dengue serotype 2 virus into EAhy926 cells
    Fan, Dongying
    Wu, Na
    Zhang, Jing
    Wang, Zhaoyang
    Wang, Peigang
    Gao, Na
    An, Jing
    MOLECULAR BIOLOGY REPORTS, 2020, 47 (12) : 9739 - 9747
12345