Domain Ⅲ of Dengue Virus Serotype 2 Envelope: Expression at High Levels in Escherichia coli and Competitive Inhibition of Virus Entry

被引：0
|
作者：
Yong-hong Yang [1 ]
Xue-li Zheng [1 ]
Lei-zi Qing [1 ]
Pin-ting Zhu [1 ]
Jing Pan [1 ]
Lei Luo [2 ]
机构：
[1] Department of Etiobiology of School of Public Health and Tropical Medicine of South Medical University
[2] Guangzhou Center for Disease Control and Prevention,No.1, Qide Road of Baiyun District
来源：
Infection International(Electronic Edition) | 2013年 / 2卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Dengue virus; Envelope domain Ⅲ; Virus entry; Vaccine; Neutralizing antibodies;
D O I：
暂无
中图分类号：
R373 [人体病毒学（致病病毒）];
学科分类号：
100103 ; 100705 ;
摘要：
Obejective The domainⅢof dengue virus type 2 envelope was cloned and expressed in Escherichia coli and the inhibited effects of recombinant protein on virus was detected.Methods In this study,the domainⅢ(DⅢ)protein of the dengue virus type-2(DENV-2)envelope(E)antigen was expressed in Escherichia coli by fusion with a carrier protein.The protein was purified using enzymatic cleavage and affinity purification.Rabbit immunization and antibody detection was carried out.Inhibition of DENV-2 infection was observed by DENV-2 EDⅢprotein and its immunity rabbits serum.Results The recombinant expression DENV-2 EDⅢprotein plasmid was constructed successfully.After isopropyl thiogalactoside induction,a specific soluble 29 kD protein was obtained,and the expression product
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页码：114 / 122
页数：9
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