Effects of derivatives of human glycophorin A and their antibodies on the recognition and invasion of Plasmodium falciparum into erythrocytes

Electrophoresis-purified human glycophorin A(GPA)was used to produce its derivatives:(1)separation and purification of glycopeptides from GPA were performed after trypsin-digestion:(2)prepanation of GPA antibody and GPA glycopeptide antibody;(3)preparation of deglycosylatedGPA(dGPA);(4)incorporating GPA or dGPA into human RBC membrane lipids to form twokinds of liposomes.The products described above were used to test Plasmodim falciparumFCC-1/HN merozoites for their ability to invade human erythrocytes.It was found that GPA-liposomes were able to bind with merozoites and dGPA-liposomes had a negative reaction.GPA,GPA glycopeptide,GPA antibody,GPA glycopeptide antibody and GPA-liposome all had the effectto hinder the invasion of merozoites into human erythrocyte,whereas dGPA-liposome had no suchan effect.