Proteomic Analysis of the Response of Liangyoupeijiu(Super High-Yield Hybrid Rice) Seedlings to Cold Stress

Liangyoupeijiu is a super high-yield hybrid rice.Despite its advantages with respect to yield and grain quality,it is sensitive to cold,which keeps it from being widely cultivated.We subjected Liangyoupeijiu seedlings to 4℃cold treatment,then extracted the leaf proteins.After 2-D gel electrophoresis separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis,a series of differentially displayed proteins were identified.Some metabolism-associated proteins were found among the downregulated proteins,such as carbamoyl phosphate synthetase,transketolase 1,dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase.The upregulated proteins included both stress-resistance proteins such as nucleoside diphosphate kinaseⅠand proteins that are negative for rice growth,such as FtsH-like protein,plastid fusion and/or translocation factor(Pftf) and actin.Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolic activity and damaging cell structure.