Ovalbumin-stabilized gold nanoclusters with ascorbic acid as reducing agent for detection of serum copper

被引：0

作者：

Yifan Chen ^{[1
,2
]}

Juan Qiao ^{[1
,3
]}

Qianrong Liu ^{[1
,4
]}

Li Qi ^{[1
,3
]}

机构：

[1] Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry,Chinese Academy of Sciences

[2] Zhuhai College of Jilin University

[3] University of Chinese Academy of Sciences

[4] Xi'an University of Science and Technology

来源：

ChineseChemicalLetters | 2018年 / 29卷 / 03期

关键词：

Ovalbumin-stabilized gold nanoclusters; Fluorescent probe; Ascorbic acid; Reducing agent; Serum copper;

D O I：

暂无

中图分类号：

O657.3 [光化学分析法（光谱分析法）]; TB383.1 [];

学科分类号：

070302 ; 081704 ; 070205 ; 080501 ; 1406 ;

摘要：

Ovalbumin-stabilized gold nanoclusters（OVA@AuNCs） were prepared with ascorbic acid as a reducing agent. This strategy could realize the synthesis of water-soluble OVA@AuNCs within 20 min. The asprepared fluorescent probe showed a red fluorescence emission at 630 nm. Moreover, the properties of the OVA@AuNCs were characterized by transmission electron microscope, dynamic light scattering,ultraviolet-visible spectroscopy, fluorescent spectroscopy. Based on the surface electron density decrease-induced fluorescence quenching mechanism, the OVA@AuNCs provided high sensitivity and selectivity for sensing copper ions. A good linear relationship was obtained between the fluorescence intensity of OVA@AuNCs and the concentration of copper ions in the range of 5.0-100.0 μmol/L（R2=0.999） with a detection limit of 640 nmol/L Furthermore, the rat serum copper contents were determined by using the OVA@AuNCs based assay, indicating great potential of fluorescent probes for application in biological and clinical analysis.

引用

页码：366 / 370

页数：5