Effect of SJAMP on Human Platelet Cytoplasmic Ca2+

Using the method of dual-wavelength measurement of platelet [Ca]and Fnra-2 as the Cafluorophore probe, we measured the effect of acidic Mu copolysaccharide front Sticopus Japonicus Selenka (SJAMP) on platelet [Ca].The results showed that the most significant increase in platelets [Ca]was seen when the concentration of SJAMP wits 100μg/ml and the elevation of normal platelet [Ca]was 93.96±10.24 nmol/L (n=10). In the presence of extracellular Ca(1 mmol/L), the magnitude of platelet [Ca]response to SJAMP was increased and the [Ca]could reach 116.72±10.66 nmol/L (n=10). On the other hand, the magnitude of increased platelet [Ca]induced by SJAMP was smaller and the duration of [Ca]reaching the highest level was longer when compared with other platelet aggregation agents. In the mean time, if platelets were first incubated with cyclooxygenase inhibitor, the rise of [Ca]evoked by SJAMP was inhibited. The results indicated that