The effect of cytoplasmic regions of LIF receptor on activating MAPK p42/44in HL-60 cell

被引：0

作者：

刘厚奇

单继宽

汤淑萍

刘善荣

冀凯宏

机构：

来源：

Journal of Medical Colleges of PLA | 2001年 / 03期

关键词：

cytokine; receptor; mitogen-activatedproteinkinase; HL-60;

D O I：

暂无

中图分类号：

R392.1 [免疫生物学];

学科分类号：

100102 ;

摘要：

Objective:To studytherelationbetweenthecytoplasmicregionof eachsubunitof leukemiainhibitoryfactor(LIF)receptorandmitogen-activatedproteinkinase(MAPK)in humanleukemialineHL-60for studyingthe mechanism of leukemiacellproliferationdisorder.Methods:Wepreparedthechimericalreceptorwithexchangedcytoplasmicdo-main(190/130,130/190),expressediton themembraneof HL-60,andthenboundLIFwiththewildtypereceptorcompet-itively.Thecytoplasmicregionhomodimerization(190cyt-190cyt,130cyt-130cyt)initiatedintracellularsignaltransduc-tion,cellproliferationanddifferentiation.MAPKphosphorylationlevelwasobservedby meansof immunoblottingand immunobiochemistry.Results:Higherlevelof MAPKp42/p44phosphorylation(Thr202Tyr204)andfastercellproliferation weredeterminedingroup190/130comparedwiththewildtypereceptor.Thoseingroup130/190werelowerthantheoth-ers.Conclusion:Thecytoplasmicdomainof gp130involvesin theinductionof MAPKactivationandthecellprolifera-tionof HL-60.

引用

页码：211 / 213 +219

页数：4

共 19 条

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Houqi Liu
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Molecular and Cellular Biochemistry, 2001, 217 : 113 - 120
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