Human umbilical cord blood-derived mesenchymal stem cells promote regeneration of crush-injured rat sciatic nerves

被引：0

作者：

MiAe Sung ^{[1
]}

Hun Jong Jung ^{[2
]}

JungWoo Lee ^{[3
]}

JinYong Lee ^{[3
]}

Sang Bae Yoo ^{[4
]}

Mohammad SAlrashdan ^{[3
]}

SoungMin Kim ^{[3
]}

Jeong Won Jahng ^{[4
]}

JongHo Lee ^{[5
,3
,4
]}

机构：

[1] Department of Craniofacial Structure&Functional Biology, School of Dentistry,Seoul National University

[2] Department of Occupation and Environment,Postgraduate College of Medicine,Konkuk University

[3] Department of Oral&Maxillofacial Surgery,School of Dentistry,Seoul National University

[4] Dental Research Institute,Seoul National University

[5] Department of Craniofacial Structure&Functional Biology,School of Dentistry,Seoul National University

来源：

NeuralRegenerationResearch | 2012年 / 7卷 / 26期

关键词：

human umbilical cord blood-derived mesenchymal stem cells; sciatic nerve; crush injury; Fluoro Gold; stem cells; peripheral nerve regeneration; regeneration; neural regeneration;

D O I：

暂无

中图分类号：

R745 [周围神经及神经节疾病];

学科分类号：

1002 ;

摘要：

Several studies have demonstrated that human umbilical cord blood-derived mesenchymal stem cells can promote neural regeneration following brain injury. However, the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells in guiding peripheral nerve regeneration remain poorly understood. This study was designed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells on neural regeneration using a rat sciatic nerve crush injury model. Human umbilical cord blood-derived mesenchymal stem cells (1 × 10 6 ) or a PBS control were injected into the crush-injured segment of the sciatic nerve. Four weeks after cell injection, brain-derived neurotrophic factor and tyrosine kinase receptor B mRNA expression at the lesion site was increased in comparison to control. Furthermore, sciatic function index, Fluoro Gold-labeled neuron counts and axon density were also significantly increased when compared with control. Our results indicate that human umbilical cord blood-derived mesenchymal stem cells promote the functional recovery of crush-injured sciatic nerves.

引用

页码：2018 / 2027

页数：10

共 18 条

[1] Effect of controlled co-delivery of synergistic neurotrophic factors on early nerve regeneration in rats [J].

Madduri, Srinivas ;

di Summa, Pietro ;

Papaloizos, Michael ;

Kalbermatten, Daniel ;

Gander, Bruno .

BIOMATERIALS, 2010, 31 (32) :8402-8409

[2]

OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells （hUCB–MSCs）[J] . Kwang-Won Seo,Sae-Rom Lee,Dilli Ram Bhandari,Kyoung-Hwan Roh,Sang-Bum Park,Ah-Young So,Ji-Won Jung,Min-Soo Seo,Soo-Kyung Kang,Yong-Soon Lee,Kyung-Sun Kang.Biochemical and Biophysical Research Communications . 2009 (1)

[3] Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model [J].

Sun, Bo ;

Roh, Kyoung-Hwan ;

Park, Jeong-Ran ;

Lee, Sae-Rom ;

Park, Sang-Bum ;

Jung, Ji-Won ;

Kang, Soo-Kyung ;

Lee, Yong-Soon ;

Kang, Kyung-Sun .

CYTOTHERAPY, 2009, 11 (03) :289-U14

[4]

Cord Blood Stem Cells: A Review of Potential Neurological Applications[J] . David T. Harris.Stem Cell Reviews . 2008 (4)

[5]

Species and strain differences in rodent sciatic nerve anatomy: Implications for studies of neuropathic pain[J] . Marcel Rigaud,Geza Gemes,Marie-Elizabeth Barabas,Donna I. Chernoff,Stephen E. Abram,Cheryl L. Stucky,Quinn H. Hogan.Pain . 2008 (1)

[6] The potential of umbilical cord blood multipotent stem cells for nonhematopoietic tissue and cell regeneration [J].

van de Ven, Carniella ;

Collins, Daniel ;

Bradley, M. Brigid ;

Morris, Erin ;

Cairo, Mitchell S. .

EXPERIMENTAL HEMATOLOGY, 2007, 35 (12) :1753-1765

[7] Human umbilical cord blood cells express neurotrophic factors [J].

Fan, CG ;

Zhang, QJ ;

Tang, FW ;

Han, ZB ;

Wang, GS ;

Han, ZC .

NEUROSCIENCE LETTERS, 2005, 380 (03) :322-325

[8] Infusion of human umbilical cord blood cells in a rat model of stroke dose-dependently rescues behavioral deficits and reduces infarct volume [J].

Vendrame, M ;

Cassady, J ;

Newcomb, J ;

Butler, T ;

Pennypacker, KR ;

Zigova, T ;

Sanberg, CD ;

Sanberg, PR ;

Willing, AE .

STROKE, 2004, 35 (10) :2390-2395

[9] Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration [J].

Tohill, M ;

Mantovani, C ;

Wiberg, M ;

Terenghi, G .

NEUROSCIENCE LETTERS, 2004, 362 (03) :200-203

[10] Transplantation of human umbilical cord blood cells in the repair of CNS diseases [J].

Newman, MB ;

Davis, CD ;

Borlongan, CV ;

Emerich, D ;

Sanberg, PR .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2004, 4 (02) :121-130

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