Mechanism of multi-point site-directed mutagenesis

被引：0
|
作者：
谢毅
肖谷田
林卿
王顺德
叶韫辉
吴小舟
机构：
来源：
Progress in Natural Science | 1997年 / 03期
关键词：
site-directed mutagenesis; multi-point site-specific mutagenesis; protein engineering; subtilisin E;
D O I：
暂无
中图分类号：
Q819 [生物工程应用];
学科分类号：
0836 ; 090102 ;
摘要：
Subtilisin E gene was mutagemzed in vitro as a model in one-round mutagenesis with multiple chemical-synthesized mutagenic oligonucleotides. The rationale of the new method is suggested The elements that influence the mutant efficiency such as the type, amount, distribution and mutual location of primers, and the primer to template molar ratio are discussed
引用
收藏
页码：48 / 55
页数：8
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