Effects of hydrogen-rich saline on platelet activation in hypertensive rats

OBJECTIVE To investigate the effect of hydrogen-rich saline on platelet activation in hypertensive rats.METHODS The male Wistar rats were divided into control group,hypertension group,control+hydrogen-rich saline group and hypertension+hydrogen-rich saline group.Hypertension was induced by subcutaneous infusion with angiotensinⅡ0.7 mg·kg-1·d-1for 2 weeks by osmotic mini-pumps in rats.Hydrogen-rich saline(10 mL·kg-1·d-1)was administered by intraperitoneal injection for 14 d.Platelet adhesion on collagen surface was evaluated using a well-defined perfusion chamber at low shear rate(300s-1)and high shear rate(1080s-1).The maximum aggregation rate of platelets induced by ADP was determined by turbidimetry.The levels of reactive oxygen species(ROS),nitric oxide(NO)and Ca2+in platelets were measured with flow cytometry.RESULTS When compared with the control group,platelet aggregation,platelet adhesion rate in high shear rate and low shear rate,and the level of ROS and Ca2+in platelets were elevated in hypertensive group.However,NO level in platelets decreased.Compared with the hypertensive group,hydrogen-rich saline treatment decreased platelet aggregation,platelet adhesion rate,the levels of ROS and Ca2+in platelets,and increased NO level in platelets of hypertensive rats.CONCLUSION Hydrogen-rich saline could inhibit platelet activation in hypertensive rats.This effect may be related to antioxidative stress.