马抗SARS-CoV-2特异性IgG与F(ab’)2的制备研究

被引：1

作者：

许梦园 ^{[1
,2
]}

毕津豪 ^{[2
,3
]}

王馨悦 ^{[2
,4
]}

张颖 ^{[2
,5
]}

韩秋雪 ^{[2
,6
]}

莫若 ^{[2
,3
]}

闫飞虎 ^{[2
]}

杨松涛 ^{[2
,1
,3
]}

冯娜 ^{[2
]}

王铁成 ^{[2
]}

赵永坤 ^{[2
]}

夏咸柱 ^{[2
,1
,3
]}

机构：

[1] 吉林大学动物医学学院

[2] 中国农业科学院长春兽医研究所

[3] 吉林农业大学动物医学院

[4] 东北农业大学动物医学学院

[5] 东北林业大学野生动物与自然保护地学院

[6] 北京协和医学院比较医学中心

来源：

中国兽医科学 | 2022年 / 52卷 / 07期

关键词：

新型冠状病毒; 马免疫球蛋白; F(ab’)2; 亲和层析; 假病毒中和试验;

D O I：

10.16656/j.issn.1673-4696.2022.0090

中图分类号：

S852.65 [家畜病毒学];

学科分类号：

090601 ;

摘要：

收集经SARS-CoV-2病毒样颗粒（VLPs）为免疫原免疫的健康马血清，采用亲和层析法分离血清中总IgG，利用胃蛋白酶消化Fc片段获得免疫球蛋白F（ab’）2，将二者使用结合RBD蛋白的免疫层析柱进行纯化，获得具有高度特异性的马抗SARS-CoV-2 IgG与免疫球蛋白F（ab’）2。结果显示，特异性IgG与F（ab’）2浓度是2.36 mg/mL和1.05 mg/mL，收率为11%和4.89%，纯度在91%与96%以上，对假病毒的半抑制浓度（IC50）为1.406μg/mL和0.862μg/mL。结果表明，成功制备了纯度高、特异性强且具有中和活性的马抗SARS-CoV-2特异性IgG与特异性F（ab’）2，为制备安全高效的抗SARS-CoV-2治疗性抗体药物奠定了基础。

引用

页码：846 / 852

页数：7

共 26 条

[1]

High Efficacy of Therapeutic Equine Hyperimmune Antibodies Against SARS-CoV-2 Variants of Concern.[J].Moreira Soto Andres;Arguedas Mauricio;Brenes Hebleen;Buján Willem;Corrales Aguilar Eugenia;Díaz Cecilia;Echeverri Ann;Flores Díaz Marietta;Gómez Aarón;Hernández Andrés;Herrera María;León Guillermo;Macaya Román;Kühne Arne;Molina Mora José Arturo;Mora Javier;Sanabria Alfredo;Sánchez Andrés;Sánchez Laura;Segura Álvaro;Segura Eduardo;Solano Daniela;Soto Claudio;Stynoski Jennifer L.;Vargas Mariá

[2] Broad sarbecovirus neutralization by a human monoclonal antibody [J].

Tortorici, M. Alejandra ;

Czudnochowski, Nadine ;

Starr, Tyler N. ;

Marzi, Roberta ;

Walls, Alexandra C. ;

Zatta, Fabrizia ;

Bowen, John E. ;

Jaconi, Stefano ;

Di Iulio, Julia ;

Wang, Zhaoqian ;

De Marco, Anna ;

Zepeda, Samantha K. ;

Pinto, Dora ;

Liu, Zhuoming ;

Beltramello, Martina ;

Bartha, Istvan ;

Housley, Michael P. ;

Lempp, Florian A. ;

Rosen, Laura E. ;

Dellota, Exequiel, Jr. ;

Kaiser, Hannah ;

Montiel-Ruiz, Martin ;

Zhou, Jiayi ;

Addetia, Amin ;

Guarino, Barbara ;

Culap, Katja ;

Sprugasci, Nicole ;

Saliba, Christian ;

Vetti, Eneida ;

Giacchetto-Sasselli, Isabella ;

Fregni, Chiara Silacci ;

Abdelnabi, Rana ;

Foo, Shi-Yan Caroline ;

Havenar-Daughton, Colin ;

Schmid, Michael A. ;

Benigni, Fabio ;

Cameroni, Elisabetta ;

Neyts, Johan ;

Telenti, Amalio ;

Virgin, Herbert W. ;

Whelan, Sean P. J. ;

Snell, Gyorgy ;

Bloom, Jesse D. ;

Corti, Davide ;

Veesler, David ;

Pizzuto, Matteo Samuele .

NATURE, 2021, 597 (7874) :103-+

[3] Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors [J].

Mor, Michael ;

Werbner, Michal ;

Alter, Joel ;

Safra, Modi ;

Chomsky, Elad ;

Lee, Jamie C. ;

Hada-Neeman, Smadar ;

Polonsky, Ksenia ;

Nowell, Cameron J. ;

Clark, Alex E. ;

Roitburd-Berman, Anna ;

Ben-Shalom, Noam ;

Navon, Michal ;

Rafael, Dor ;

Sharim, Hila ;

Kiner, Evgeny ;

Griffis, Eric R. ;

Gershoni, Jonathan M. ;

Kobiler, Oren ;

Leibel, Sandra Lawrynowicz ;

Zimhony, Oren ;

Carlin, Aaron F. ;

Yaari, Gur ;

Dessau, Moshe ;

Gal-Tanamy, Meital ;

Hagin, David ;

Croker, Ben A. ;

Freund, Natalia T. .

PLOS PATHOGENS, 2021, 17 (02)

[4]

Advances in gene-based vaccine platforms to address the COVID-19 pandemic.[J].Pushparajah Deborah;Jimenez Salma;Wong Shirley;Alattas Hibah;Nafissi Nafiseh;Slavcev Roderick A..Advanced Drug Delivery Reviews.2021, prepublish

[5] Development of multi-specific humanized llama antibodies blocking SARS-CoV-2/ACE2 interaction with high affinity and avidity [J].

Dong, Jianbo ;

Huang, Betty ;

Jia, Zhejun ;

Wang, Bo ;

Kankanamalage, Sachith Gallolu ;

Titong, Allison ;

Liu, Yue .

EMERGING MICROBES & INFECTIONS, 2020, 9 (01) :1034-1036

[6] Recent advances in vaccine and immunotherapy for COVID-19 [J].

Rabaan, Ali A. ;

Al-Ahmed, Shamsah H. ;

Sah, Ranjit ;

Al-Tawfiq, Jaffar A. ;

Al-Qaaneh, Ayman M. ;

Al-Jamea, Lamiaa H. ;

Woodman, Alexander ;

Al-Qahtani, Manaf ;

Haque, Shafiul ;

Harapan, Harapan ;

Bonilla-Aldana, D. Katterine ;

Kumar, Pavan ;

Dhama, Kuldeep ;

Rodriguez-Morales, Alfonso J. .

HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2020, 16 (12) :3011-3022

[7] Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells [J].

Cao, Yunlong ;

Su, Bin ;

Guo, Xianghua ;

Sun, Wenjie ;

Deng, Yongqiang ;

Bao, Linlin ;

Zhu, Qinyu ;

Zhang, Xu ;

Zheng, Yinghui ;

Geng, Chenyang ;

Chai, Xiaoran ;

He, Runsheng ;

Li, Xiaofeng ;

Lv, Qi ;

Zhu, Hua ;

Deng, Wei ;

Xu, Yanfeng ;

Wang, Yanjun ;

Qiao, Luxin ;

Tan, Yafang ;

Song, Liyang ;

Wang, Guopeng ;

Du, Xiaoxia ;

Gao, Ning ;

Liu, Jiangning ;

Xiao, Junyu ;

Su, Xiao-dong ;

Du, Zongmin ;

Feng, Yingmei ;

Qin, Chuan ;

Qin, Chengfeng ;

Jin, Ronghua ;

Xie, X. Sunney .

CELL, 2020, 182 (01) :73-+

[8] The COVID-19 pandemic [J].

Ciotti, Marco ;

Ciccozzi, Massimo ;

Terrinoni, Alessandro ;

Jiang, Wen-Can ;

Wang, Cheng-Bin ;

Bernardini, Sergio .

CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES, 2020, 57 (06) :365-388

[9] Structural basis of receptor recognition by SARS-CoV-2 [J].

Shang, Jian ;

Ye, Gang ;

Shi, Ke ;

Wan, Yushun ;

Luo, Chuming ;

Aihara, Hideki ;

Geng, Qibin ;

Auerbach, Ashley ;

Li, Fang .

NATURE, 2020, 581 (7807) :221-+

[10] A Novel Coronavirus from Patients with Pneumonia in China, 2019 [J].

Zhu, Na ;

Zhang, Dingyu ;

Wang, Wenling ;

Li, Xingwang ;

Yang, Bo ;

Song, Jingdong ;

Zhao, Xiang ;

Huang, Baoying ;

Shi, Weifeng ;

Lu, Roujian ;

Niu, Peihua ;

Zhan, Faxian ;

Ma, Xuejun ;

Wang, Dayan ;

Xu, Wenbo ;

Wu, Guizhen ;

Gao, George F. ;

Tan, Wenjie .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 382 (08) :727-733

← 1 2 3 →