Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus

被引：2

作者：

Piyathida Pongsiri ^{[1
,2
]}

Kesmanee Praianantathavorn ^{[3
]}

Apiradee Theamboonlers ^{[4
]}

Sunchai Payungporn ^{[5
,6
,7
]}

Yong Poovorawan ^{[8
]}

机构：

[1] Center of Excellence in Clinical Virology

[2] Department of Pediatrics,Faculty of Medicine.Chulalongkorn University,Bangkok 10330,Thailand

[3] Department of Biochemistry,Faculty of Medicine,Chulalongkorn University,Bangkok 10330,Thailand

[4] Center of Excellence in Clinical Virology,Department of Pediatrics,Faculty of Medicine.Chulalongkorn University,Bangkok 10330,Thailand

[5] Department of Biochemistry

[6] Faculty of Medicine,Chulalongkorn University 7. Bangkok 10330,Thailand

[7] Center of Excellence in Clinical Virology,Department of Pediatries,Faculty of Medicine,Chulalongkorn University,Bangkok 10330,Thailand

来源：

Asian Pacific Journal of Tropical Medicine | 2012年 / 05期

关键词：

Multiplex; Real-time RT-PCR; Chikungunya virus; Dengue virus;

D O I：

暂无

中图分类号：

R440 [];

学科分类号：

100208 ;

摘要：

Objective:To develop diagnostic test for detection chikungunya virus(CHIKV and Dengue virus (DENV) infection.Methods:We have performed a rapid,accurate laboratory confirmative method to simultaneously detect,quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.Results:The assay’s sensitivity was 97.65%,specificity was 92.59% and accuracy was 95.82%when compared to conventional RT-PCR.Additionally,there was no cross-reaction between CHIKV,DENV,Japanese encephalitis virus,hepatitis C,hepatitis A or hepatitis E virus.Conclusions:This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.

引用

页码：342 / 346

页数：5

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