Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2

被引：0

作者：

Hue Thi NGUYEN ^{[1
]}

Quang Hong VU ^{[1
]}

Tan Van MAI ^{[1
]}

Thu Thi NGUYEN ^{[1
]}

Lam Duc VU ^{[1
]}

Tung Thanh NGUYEN ^{[1
]}

Long Viet NGUYEN ^{[2
]}

Hien Thu Thi VU ^{[2
]}

Hue Thi NONG ^{[3
]}

Trung Nguyen DINH ^{[4
]}

Nakano TOSHITSUGU ^{[5
]}

Liet Van VU ^{[1
]}

机构：

[1] Crop Research and Development Institute, Vietnam National University of Agriculture

[2] Faculty of Agronomy, Vietnam National University of Agriculture

来源：

Rice Science | 2018年 / 01期

关键词：

rice; backcrossing; bacterial leaf blight; marker-assisted selection;

D O I：

暂无

中图分类号：

S435.111.47 [];

学科分类号：

090401 ; 090402 ;

摘要：

Bacterial leaf blight of rice （BLB）, caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent.IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC;F;lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines （BC;F;5.1.5.1, BC;F;5.1.5.12, BC;F;8.5.6.44, BC;F;9.5.4.1 and BC;F;9.5.4.23） were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security.

引用

页码：52 / 56

页数：5

共 23 条

[1] Genotypic and Pathotypic Diversity in Xanthomonas oryzae pv. oryzae in Nepal. Adhikari T B,Mew T W,Leach J E. Phytopathology . 2008
[2] A simplified assay for milled rice amylose. Juliano BO. Cereal Science Today . 1971
[3] Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21[J] . Pamela C. Ronald,Beng Albano,Rodante Tabien,Lleva Abenes,Kung-sheng Wu,Susan McCouch,Steven D. Tanksley. &nbspMGG Molecular & General Genetics . 1992 (1)
[4] Markers for selection of the rice Xa21 disease resistance gene
Williams, CE
Wang, B
Holsten, TE
Scambray, J
daSilva, FDG
Ronald, PC
[J]. THEORETICAL AND APPLIED GENETICS, 1996, 93 (07) : 1119 - 1122
[5] Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR[J] . N. Huang,E. R. Angeles,J. Domingo,G. Magpantay,S. Singh,G. Zhang,N. Kumaravadivel,J. Bennett,G. S. Khush. &nbspTAG Theoretical and Applied Genetics . 1997 (3)
[6] Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106[J] . S. Singh,J. S. Sidhu,N. Huang,Y. Vikal,Z. Li,D. S. Brar,H. S. Dhaliwal,G. S. Khush. &nbspTAG Theoretical and Applied Genetics . 2001 (6-7)
[7] PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT. Oliver H. Lowry,Nira J. Rosebrough,A. Lewis Farr,Rose J. R,a. Journal of Biological Chemistry . 1951
[8] Positional cloning of rice semidwarfing gene, sd-1: rice "green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis. Monna L,Kitazawa N,Yoshino R,et al. DNA Research . 2002
[9] A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Song W Y,Wang G L,Chen L L,et al. Science . 1995
[10] Xanthomonas oryzae pathovars: model pathogens of a model crop[J] . &nbspMolecular Plant Pathology . 2006 (5)

← 1 2 3 →