Onco-microRNA miR-130b promoting cell growth in children APL by targeting PTEN

被引:0
作者
Xiang-Cui Gong [1 ]
Yuan-Qin Xu [1 ]
Yan Jiang [1 ]
Hui Guan [1 ]
Hua-Lin Liu [1 ]
机构
[1] Department of Hematology,Affiliated Hospital of Medical College of Qingdao University
关键词
MicroRNA-130b; Acute promyelocytic leukemia; PTEN; Bcl-2; Bax; Cell growth;
D O I
暂无
中图分类号
R733.71 [急性白血病];
学科分类号
100214 ;
摘要
Objective:To study the expression of microRNA-130b(miR-l 30b) in children acute promyelocytic leukemia(APL) and its role for regulating PTEN expression.Methods:A total of SO children APL marrow tissues and IS normal marrow tissues between January and December in 2012 were collected into our study.The expression of miR-l30 b in APL and normal marrow tissues were detected by quantitative real-time polymerase chain reaction.MiR-l30 b inhibitor was transfected into HL-60 cells.Cell Counting Kit-8 assay and flow cytometry were used to measure cell proliferation and apoptosis.respectively.The expression of PTEN,a potential target of miR-130 b,and its downstream genes,Bcl-2 and Box,in transformed cells were detected by quantitative real-time polymerase chain reaction and western-blot Results:The expression of miR-l30 b was significantly higher in children APL marrow tissues than in normal marrow tissues(P<0.05).Down-regulation of miR-1 30 b could significantly suppress cell proliferation and induce apoptosis in HL-60 cells(P<0.05).PTEN expression was upregulated when miR-130 b was knocking-down(P<0.05).As downstream genes of PTEN,the expression of Bcl-2 and Box were regulated as well.Conclusions:MiR-130 b is overexpressed in children APL marrow tissues and associated with cell growth.MiR-130 b may promote children APL progression by inducing cell proliferation and inhibiting apoptosis.
引用
收藏
页码:260 / 263
页数:4
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