狭叶薰衣草的离体培养及挥发性成分的分析

被引：5

作者：

杨鑫 ^{[1
]}

詹爽 ^{[1
]}

彭尽晖 ^{[2
]}

彭豹 ^{[1
]}

郑亚杰 ^{[2
]}

彭晓英 ^{[1
]}

机构：

[1] 不详

[2] 湖南农业大学生物科学技术学院

[3] 不详

[4] 湖南农业大学园艺学院

[5] 不详

来源：

热带亚热带植物学报 | 2020年 / 01期

关键词：

狭叶薰衣草; 离体培养; 挥发油; 气相色谱-质谱;

D O I：

暂无

中图分类号：

S567.239 [];

学科分类号：

摘要：

为建立新疆狭叶薰衣草(Lavandula angustifolia)的快速繁殖体系,以种子、茎、叶为外植体,对种子萌发、愈伤组织诱导、丛芽分化和生根的最适培养条件进行了研究;用水蒸气蒸馏法提取狭叶薰衣草挥发油,采用气相色谱-质谱法测定挥发油成分。结果表明,种子浸泡的适宜时间为6 h,切开种皮培养,出芽时间最少为6 d;诱导种子出芽的适宜培养基为MS+6-BA2 mg/L;以茎为外植体诱导愈伤组织效果较好,适宜培养基为MS+6-BA 2 mg/L+2,4-D 1 mg/L;诱导分化丛芽的适宜培养基为MS+6-BA 1 mg/L+NAA 0.5 mg/L;生根的适宜培养基为1/2MS+NAA 1 mg/L+6-BA 0.5 mg/L;盆栽薰衣草和无菌苗薰衣草的挥发油主要成分相差较大,离体培养的薰衣草的主要挥发性成分有叶绿醇、丁香油烃、氧化石竹烯等。

引用

页码：84 / 90

页数：7

共 25 条

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