IcarisideⅡ inhibits lipopolysaccharide-induced inflammation and amyloid production in astrocytes by regulating IKK/IκB/NF-κB signaling pathway

OBJECTIVE To investigate the effect of icariin Ⅱ（ICS Ⅱ） on lipopolysaccharide（LPS）-induced inflammation and amyloid production in astrocytes.METHODS The cerebral cortex of newborn SD rats was isolated in vitro,and the primary astrocytes were extracted and cultured.Astrocytes were pre-treated with ICSⅡ(5,10 and20 μmol·L;) or dexamethasone(1 μmol·L;) for1 h.Cell inflammation models were established with LPS and treated with ICS Ⅱ or dexamethasone for another 24 h.The anti-neuroinflammation and anti-amyloid effects of ICS Ⅱ in astrocytes were detected by ELISA and Western blotting respectively.RESULTS ICS Ⅱ decreased the levels of beta secretase 1（BACE1）,Aβ1-40 and Aβ1-42 in astrocytes in a concentration-dependent manner.Moreover,the levels of tumor necrosis factor-alpha,interleukin-1β,reactive oxygen species,inducible nitric oxide synthase,cyclooxygenase-2 and transforming growth factor-β1 in astrocytes were significantly inhibited by ICS II(5,10 and 20 μmol·L;).In addition,ICSⅡhas a significant inhibitory effect on LPS-induced IκB-α degradation and NF-κB activation.CONCLUSION ICS Ⅱ exerts neuroprotective effects on LPS-induced inflammation in astrocytes,through regulating IKK/IκB/NF-κB signaling pathway.