Optimization strategies for separation of sulfadiazines using Box-Behnken design by liquid chromatography and capillary electrophoresis

被引：0

作者：

龚文君 ^{[1
]}

张裕平 ^{[1
]}

张毅军 ^{[1
]}

许光日 ^{[1
]}

魏新军 ^{[1
]}

LEE Kwangpill ^{[2
]}

机构：

[1] Henan Institute of Science and Technology

[2] Department of Chemistry, Graduate School, Kyungpook National University, South Korea

来源：

Journal of Central South University of Technology | 2007年 / 02期

关键词：

Box-Behnken design; high performance liquid chromatography; capillary electrophoresis; sulfadiazine; multi-criteria decision;

D O I：

暂无

中图分类号：

O658.9 [其他];

学科分类号：

070302 ; 081704 ;

摘要：

Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for the separation of interested components. Box-Behnken factorial design was effectively applied for the separation optimization of eight structurally related sulfonamides using capillary zone electrophorosis and reverse high performance liquid chromatography. Optimum values for volume ratio of THF to H2O in eluent, column temperature and flow rate of eluent are found as 12 to 88, 35℃ and 1.0 mL/min, respectively. Box-Behnken modified optimization model is extended to separation by capillary electrophoresis (CE). While using CE, a satisfactory separation is achieved with a minimum resolution larger than 1.0 for a separation time less than 10 min.

引用

页码：196 / 201

页数：6