Application of Phage-displayed Single Chain Antibodies in Western Blot

A phage display single chain fragment variable library constructed on pⅢ protein of M13filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules.After fourrounds of panning,there was a remarkable enrichment in the titer of bound phages.Twenty phage cloneswere selected from the last round and screened by means of phage-ELISA.With the antibody phages asprimary antibodies in Western blot,we developed a method for detecting the specific antigen.The dilutions ofantibody phages depend on the affinity between antibody-displayed phage particles and antigens.