Application of Phage-displayed Single Chain Antibodies in Western Blot

被引：0
|
作者：
Yan-Li DING Mei-Yun LIU Wei HAN Sheng-Li YANG Hui LIU Yi GONG School of Pharmaceutical Engineering
Shanghai Research Center of Biotechnology
机构：
来源：
Acta Biochimica et Biophysica Sinica | 2005年 / 03期
关键词：
phage-display; antibody; Western blot;
D O I：
暂无
中图分类号：
Q78 [基因工程（遗传工程）];
学科分类号：
071007 ; 0836 ; 090102 ;
摘要：
A phage display single chain fragment variable library constructed on pⅢ protein of M13filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules.After fourrounds of panning,there was a remarkable enrichment in the titer of bound phages.Twenty phage cloneswere selected from the last round and screened by means of phage-ELISA.With the antibody phages asprimary antibodies in Western blot,we developed a method for detecting the specific antigen.The dilutions ofantibody phages depend on the affinity between antibody-displayed phage particles and antigens.
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页码：205 / 209
页数：5
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