沉默GPC-3基因转录对裸鼠肝癌移植瘤的抑制作用

被引：2

作者：

陈洁

姚敏

顾星

钱琦

陆少林

姚登福

机构：

[1] 南通大学附属医院临床医学研究中心

来源：

南通大学学报(医学版) | 2013年 / 33卷 / 06期

关键词：

肝细胞性肝癌; 磷脂酰肌醇蛋白多糖-3; 基因沉默; 裸鼠; 移植瘤;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

100214 ;

摘要：

目的:研究沉默磷脂酰肌醇蛋白多糖-3（glypican-3,GPC-3）基因转录抑制肝癌HepG2细胞裸鼠移植瘤生长与机制。方法:以人GPC-3基因序列设计并合成miRNA,构建GPC-3-miRNA干扰质粒。将其导入HepG2细胞,通过杀稻瘟菌素筛选出稳定株,移植裸鼠皮下成瘤。根据体积的均数值绘制肿瘤生长曲线,免疫组化法检测瘤组织内GPC-3、β-catenin、p-GSK3β及cyclinD1表达。结果 :肝癌HepG2、Hep3B和MHCC-97H细胞GPC-3表达较强（50%80%）,SMMC-7721和Bel-7402细胞中等（18%25%）,PLC/PRF/5和Bel-7404细胞较弱（8%10%）。以特异miRNA干预GPC-3基因转录,明显抑制HepG2细胞增殖,呈时间依赖性;沉默GPC-3能明显抑制裸鼠皮下移植瘤生长,且瘤组织β-catenin、p-GSK3β及cyclinD1等关键信号分子表达降低。结论:沉默GPC-3影响Wnt/β-catenin通路关键信号分子表达可能是抑制HepG2增殖的分子机制。

引用

页码：457 / 460

页数：4

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