Cloning and expression analysis of HXK1 gene from Jatropha curcas L.

被引：0

作者：

Hu Xin ^{[1
,2
,3
]}

Lizhou Tang ^{[2
,3
]}

Chao Liu ^{[2
,3
]}

Yong Gao ^{[2
,3
]}

Dongqin Dai ^{[2
,3
]}

Huanhuan Chen ^{[2
,3
]}

Yan Deng ^{[1
]}

Bin Tian ^{[1
]}

Haibo Wang ^{[2
,3
]}

机构：

[1] Key Laboratory of Biodiversity Conservation in Southwest State Forestry Administration,Southwest Forestry University

[2] Center for Yunnan Plateau Biological Resources Protection and Utilization,Qujing Normal University

[3] Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yunnan-Guizhou Plateau,Qujing Normal University

来源：

OilCropScience | 2020年 / 5卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Jatropha curcas L; Resilience; HXK1; Gene cloning; Functional analysis;

D O I：

暂无

中图分类号：

S794.9 [其他];

学科分类号：

0829 ; 0907 ;

摘要：

HXK(Hexokinase) gene family and the role of JcHXK1 in Jatropha curcas L.were explored.Totally 4 HXK genes JcHXK1,JcHXK2, JcHXK3 and JcHKL1 were identified and complete ORF of JcHXK1 was cloned.Functional domain,phylogenetic evolution and low-temperature expression characteristics were analyzed.Results showed that full-length JcHXK1 cDNA was 1497 bp,encoding 498 amino acids with molecular weight of 53.81 kDa and pI of 5.03.Further phylogenetic evolutionary analysis demonstrated HXK1 protein was clustered into 6 small branches and 2 large branches.Sequence alignment showed that HXK1 contained several conserved glycine residues and hydrophobic channels.Prokaryotic expression vector of JcHXK1 was constructed and 12% SDS-PAGE detection showed that it was highly expressed in E.coli. These research was expected to lay a foundation for further gene functional verification and cold signal transduction mechanism for HXK1 in Jatropha curcas L.

引用

页码：85 / 89

页数：5

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