Multimetallic intra-nanogap nanozyme-mediated lateral flow immunoassay for ultrasensitive and multimode detection of K. pneumonia in clinical samples

被引：0

作者：

Zhi, Weixia ^{[1
]}

Guo, Xinjie ^{[1
]}

Nie, Yichu ^{[5
]}

Nie, Lu ^{[5
]}

He, Tingting ^{[1
]}

Dai, Li ^{[1
]}

Xu, Jing ^{[1
]}

Zong, Xiangxin ^{[1
]}

Xu, Jun ^{[1
]}

Cai, Huaihong ^{[6
]}

Tang, Yong ^{[7
]}

Cong, Yanguang ^{[3
]}

Pi, Jiang ^{[3
]}

Zhu, Youfeng ^{[1
]}

Sun, Pinghua ^{[1
,2
]}

Guo, Jialiang ^{[4
]}

Chen, Huoqiang ^{[5
]}

Huang, Xueqin ^{[3
]}

Zhou, Haibo ^{[1
,2
]}

机构：

[1] State Key Laboratory of Bioactive Molecules and Druggability Assessment, The Fifth Affiliated Hospital, Guangzhou Red Cross Hospital, College of Pharmacy, Jinan University, Guangzhou

[2] Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), School of Pharmacy, Shihezi University, Shihezi

[3] The First Dongguan Affiliated Hospital, School of Medical Technology, Guangdong Medical University, Dongguan

[4] School of Medicine, Foshan University, Foshan

[5] The First People's Hospital of Foshan, Foshan

[6] College of Chemistry and Materials Science, Jinan University, Guangzhou

[7] College of Life Science and Technology, Jinan University, Guangzhou

来源：

Chemical Engineering Journal | 2025年 / 520卷

基金：

中国国家自然科学基金;

关键词：

Klebsiella pneumonia; Lateral flow immunoassay; Nanozyme; Point-of-care testing; Surface-enhanced Raman scattering;

D O I：

10.1016/j.cej.2025.166410

中图分类号：

学科分类号：

摘要：

The current lack of rapid screening methods for Klebsiella pneumonia (K. pneumonia) poses a considerable threat to human health. Herein, we fabricated a multilayer Au@Au@Ag/Pt nanoparticle (NP) that made of the peroxidase-mimicking external shell and tunable plasmonic intra-nanogap as a versatile probe, and conjugated them with lateral flow immunoassay (LFIA) for colorimetric-catalytic-SERS (CM/CL/SERS) multi-model sensing K. pneumonia in complex biological matrix. After the optimization of several parameters, the limit of detection (LOD) of the target K. pneumonia were 103 CFU/mL for CL-LFIA and 38 CFU/mL for SERS-LFIA, both of which were over 10-fold and 200-fold more sensitive than the visual CM-LFIA (104 CFU/mL), respectively. The clinical validation of this multi-model LFIA was performed with the infected patients (N = 6) and healthy subjects (N = 3), among which 4 positive samples missed by conventional CM-LFIA were successfully identified by SERS-LFIA. More importantly, this LFIA has great specificity for the target bacteria, while showed no signal response to samples infected by other bacteria. Different from previous studies focusing on detection of protein and nucleic acid of K. pneumonia, our study shortened the sample-to-answer time with direct detection of K. pneumonia by LFIA, filling the diagnostic gap for point-of-care testing (POCT) of K. pneumonia infection. Overall, the great performance of our established LFIA suggested its substantial potential for diagnosis of K. pneumonia-induced nosocomial infection. © 2025 Elsevier B.V.

引用

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