Composition of Dithyrea wislizenii fruit extract and free-radical scavenging activity of its constituents

被引：0

作者：

Montaut, Sabine ^{[1
]}

Grandbois, Julie ^{[1
]}

Rossi, Laura S. ^{[1
]}

Kamal, Sonia ^{[1
]}

Khouri, James ^{[1
]}

Ménard, Melissa G. ^{[1
]}

Joly, Hélène A. ^{[1
]}

机构：

[1] Department of Chemistry and Biochemistry, Laurentian University, Sudbury, ON P3E 2C6

来源：

Canadian Journal of Chemistry | 2012年 / 90卷 / 08期

关键词：

Brassicaceae; Dithyrea wislizenii; flavonoids; free-radical scavengers; glucosinolates;

D O I：

10.1139/v2012-042

中图分类号：

学科分类号：

摘要：

Glucolesquerellin (2), glucohesperin (3), quercetin 3-O-sophoroside (4), and quercetin 3-O-sophoroside-7-O-glucoside (5), isolated from the fruit of Dithyrea wislizenii, were quantified by HPLC. The fruit extract and flavonoids were not found to be toxic by using a brine shrimp lethality assay. The fruit extract and the flavonoids and glucosinolates were submitted to a free-radical scavenging activity assay with the diphenylpicrylhydrazyl radical (DPPH •). The concentration of quercetin (6) (a positive control for the flavonoids) able to scavenge 50% of DPPH • (SC 50) was 32 ± 2 μmol/L (or 4 ± 1 μg/mL), which was about 27 times more potent than the crude extract. Compounds 4 and 5 had a SC 50, the concentration of the compound required to scavenge 50% of the DPPH •, of 78 ± 1 μmol/L and 113 ± 10 μmol/L, respectively. The positive control for the glucosinolates, glucoraphasatin, (1) had a SC 50 of 1768 ± 60 μmol/L. The glucosinolates 2 and 3 had a SC 50 of 7819 ± 1968 and 970 ± 63 μmol/L, respectively. © 2012 Published by NRC Research Press.

引用

页码：652 / 659

页数：7

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