Beta-Amyloid in the Optic Nerves of Young Mouse Models of Alzheimer's Disease without Clinical Symptoms

被引:0
作者
Oh, Il Joong [1 ]
Kim, Jong Heon [2 ]
Chun, Bo Young [1 ,2 ]
机构
[1] Kyungpook Natl Univ, Sch Med, Dept Ophthalmol, 130 Dongdeok Ro, Daegu 41944, South Korea
[2] Kyungpook Natl Univ, Brain Sci & Engn Inst, Daegu, South Korea
来源
JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY | 2025年 / 66卷 / 07期
关键词
Alzheimer's disease; Beta-amyloid; Mouse; Optic nerve; Retina; EYES; MICE;
D O I
10.3341/jkos.2025.66.7.305
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: This study investigated the presence of beta-amyloid in the retina and optic nerve of 2-month-old mouse models of Alzheimer's disease (AD) prior to the onset of clinical symptoms and compared the findings to those of age-matched controls. Methods: Visual acuity and contrast sensitivity were measured using an OptoDrum (R) in 2-month-old mouse models (5xFAD) and 2-month-old normal mice (C57BL/6). The retina and optic nerve of the experimental and control groups were stained fluorescently using an amyloid-beta antibody, and the level of amyloid-beta expression in the two groups was compared and analyzed histologically. Results: The 2-month-old mouse models (5xFAD) are at a stage prior to the characteristic spatial learning and cognitive impairments associated with AD. Visual acuity and contrast sensitivity did not differ between the experimental and control groups. Beta-amyloid was not observed in the retina or optic nerve of the control group or in the retina of the experimental group. In contrast to normal mice of the same age, beta-amyloid accumulated in the optic nerve in the experimental group, with a tendency for increased beta-amyloid accumulation in the optic nerve closer to the brain. Conclusions: In young AD mouse models prior to the onset of clinical symptoms, there were no differences in visual acuity, contrast sensitivity, or histological changes in the retina compared to controls. However, pathological changes in the accumulation of beta-amyloid in the optic nerve in the experimental group preceded other changes. These findings should aid the early diagnosis of AD in the future.
引用
收藏
页码:305 / 310
页数:6
相关论文
共 17 条
[11]   Intraneuronal β-amyloid aggregates, neurodegeneration, and neuron loss in transgenic mice with five familial Alzheimer's disease mutations:: Potential factors in amyloid plaque formation [J].
Oakley, Holly ;
Cole, Sarah L. ;
Logan, Sreemathi ;
Maus, Erika ;
Shao, Pei ;
Craft, Jeffery ;
Guillozet-Bongaarts, Angela ;
Ohno, Masuo ;
Disterhoft, John ;
Van Eldik, Linda ;
Berry, Robert ;
Vassar, Robert .
JOURNAL OF NEUROSCIENCE, 2006, 26 (40) :10129-10140
[12]   Introducing Amylo-Glo, a novel fluorescent amyloid specific histochemical tracer especially suited for multiple labeling and large scale quantification studies [J].
Schmued, Larry ;
Raymick, James ;
Tolleson, William ;
Sarkar, Sumit ;
Zhang, Yi-Hong ;
Bell-Cohn, Ashlee .
JOURNAL OF NEUROSCIENCE METHODS, 2012, 209 (01) :120-126
[13]   The amyloid hypothesis of Alzheimer's disease at 25years [J].
Selkoe, Dennis J. ;
Hardy, John .
EMBO MOLECULAR MEDICINE, 2016, 8 (06) :595-608
[14]  
Syed AB, 2005, FOLIA NEUROPATHOL, V43, P1
[15]   Concise Review: Making a Retina-From the Building Blocks to Clinical Applications [J].
Wallace, Valerie A. .
STEM CELLS, 2011, 29 (03) :412-417
[16]   Absence of Alzheimer Disease Neuropathologic Changes in Eyes of Subjects With Alzheimer Disease [J].
Williams, Erik A. ;
McGuone, Declan ;
Frosch, Matthew P. ;
Hyman, Bradley T. ;
Laver, Nora ;
Stemmer-Rachamimov, Anat .
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2017, 76 (05) :376-383
[17]   Computed Tomography Density and β-Amyloid Deposition of Intraorbital Optic Nerve May Assist in Diagnosing Mild Cognitive Impairment and Alzheimer's Disease: A 18F-Flutemetamol Positron Emission Tomography/Computed Tomography Study [J].
Wu, Han ;
Lei, Zhe ;
Ou, Yinghui ;
Shi, Xin ;
Xu, Qian ;
Shi, Keqing ;
Ding, Jing ;
Zhao, Qianhua ;
Wang, Xiuzhe ;
Cai, Xiaolong ;
Liu, Xueyuan ;
Lou, Jingjing ;
Liu, Xingdang .
FRONTIERS IN AGING NEUROSCIENCE, 2022, 14