Nanoscale Mapping of Multiple Lectins on Cell Surfaces by Single-Molecule Force Spectroscopy

被引：7

作者：

Cuenot, Stéphane ^{[1
]}

Bouvrée, Audrey ^{[1
]}

Bouchara, Jean-Philippe ^{[2
,3
]}

机构：

[1] Institut des Matériaux Jean Rouxel (IMN), Université de Nantes, CNRS, 2, Rue de la Houssinière, BP 32229, Nantes Cedex 3,44322, France

[2] Groupe d'Etude des Interactions Hôte-Pathogène (GEIHP, EA 3142), UNIV Angers, UNIV Brest, Université Bretagne-Loire, 4 rue Larrey, 49933, Angers cedex 9, France

[3] Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Institut de Biologie en Santé, 4 rue Larrey, Angers cedex 9,49933, France

来源：

Advanced Biosystems | 2017年 / 1卷 / 07期

关键词：

Histology - Mapping - Physiological models - Transcription - Cell membranes - Pathogens - Signal transduction - Tissue - Binding sites - Targeted drug delivery - Molecules - Plants (botany) - Adhesion - Proteins - Atomic force microscopy - Controlled drug delivery - Medical nanotechnology;

D O I：

10.1002/adbi.201700050

中图分类号：

学科分类号：

摘要：

Molecular recognition events driven by protein–carbohydrate interactions play fundamental roles in various physiological and pathological processes in living organisms, including cohesion inside tissues, innate immune response, cancer cell metastasis, and infections. Unlike widely investigated carbohydrates, detailed knowledge of both the spatial organization of specific lectins and their identification on cell surfaces remains an essential prerequisite for the understanding of pathogen adhesion to host tissues and subsequent infection prevention. In this study, the spatially resolved localization, identification, and quantification of multiple carbohydrate-binding sites are directly revealed on the surface of fungal pathogen Aspergillus fumigatus. Nanoscale reconstructed mapping from several recognition maps, corresponding each to a unique specific interaction probed by single-molecule force spectroscopy, shows the distribution of carbohydrate-binding sites on the pathogen surface. The identified binding sites are then blocked with the appropriate carbohydrate, attesting the possibility to control lectin-mediated host–pathogen interactions. Germination markedly affects both the spatial distribution of carbohydrate-binding sites, mostly expressed at the apex of hyphae, and the identity of the predominant ones, which depend on the location on germ tubes. These insights clearly open exciting avenues in nanomedicine to control host–pathogen interactions with the development of vaccines or inhibitory drugs that preferentially target the identified carbohydrate-binding sites. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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